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ARS Home » Southeast Area » Stoneville, Mississippi » Genomics and Bioinformatics Research » Research » Publications at this Location » Publication #427104
Research Project: Integrative Applied Agricultural Genomics and Bioinformatics Research

Location: Genomics and Bioinformatics Research

Title: Complete genome sequence of an umbravirus from white snakeroot (Ageratina altissima)

Author
item ABOUGHANEM-SABANADZO, NINA - Mississippi State University
item SABANADZOVIC, SEAD - Mississippi State University

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/24/2024
Publication Date: 9/12/2024
Citation: Aboughanem-Sabanadzo, N., Sabanadzovic, S. 2024. Complete genome sequence of an umbravirus from white snakeroot (Ageratina altissima). Archives of Virology. https://doi.org/10.1007/s00705-024-06125-0.
DOI: https://doi.org/10.1007/s00705-024-06125-0

Interpretive Summary: Complete genome sequencing of a virus from a white snakeroot plant (Ageratina altissima (L.) King & H. Rob.) collected in the Great Smoky Mountains National Park, USA, revealed a genome organization resembling that of umbraviruses. Sequence comparisons and phylogenetic analysis strongly suggested that this virus is a previously undefined member of the genus Umbravirus (family Tombusviridae), for which the name “white snakeroot virus A” (WSVA) is proposed. In addition, we identified and initiated characterization of its possible helper virus, a putative new member of the genus Luteovirus.

Technical Abstract: Complete genome sequencing of a virus from a white snakeroot plant (Ageratina altissima (L.) King & H. Rob.) collected in the Great Smoky Mountains National Park, USA, revealed a quadricistronic organization resembling that of umbraviruses. ORFs 1 and 2 are putatively translated via a -1 ribosomal frameshift mechanism as a single polypeptide with a role in viral replication, whereas the 3'-proximal and extensively overlapping ORFs 3 and 4 code for proteins involved in long distance trafficing and cell-to-cell movement within the host. Sequence comparisons and phylogenetic analysis strongly suggested that this virus is a previously undescribed member of the genus Umbravirus (family Tombusviridae), for which the name “white snakeroot virus A” (WSVA) is proposed. In addition, we identified and initiated characterization of its possible helper virus, a putative new member of the genus Luteovirus.