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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Quality and Safety Assessment Research Unit » Research » Publications at this Location » Publication #426640
Research Project: Smart Optical Sensing of Food Hazards and Elimination of Non-Nitrofurazone Semicarbazide in Poultry

Location: Quality and Safety Assessment Research Unit

Title: Adapting a 96-well liquid culture assay to a colonial growth confirmation assay utilizing a 3D printed guide-frame system for a 96-well replicator

Author
item Lewis, Micah
item Adams, Eric
item McMillan, Elizabeth

Submitted to: Journal of Microbiological Methods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/1/2025
Publication Date: 9/12/2025
Citation: Lewis, M.A., Adams, E.S., Mcmillan, E.A. 2025. Adapting a 96-well liquid culture assay to a colonial growth confirmation assay utilizing a 3D printed guide-frame system for a 96-well replicator. Journal of Microbiological Methods. 238, 1-4. https://doi.org/10.1016/j.mimet.2025.107246.
DOI: https://doi.org/10.1016/j.mimet.2025.107246

Interpretive Summary: A guide-frame system was developed to aid in the assessment of bacterial growth where broth applications are not suitable. The system allows the transfer of cells from the broth application to a petri dish with solid agar where growth can be easily determined.

Technical Abstract: Some bacteria are not suited to assays requiring visual growth in broth; it may be useful to transfer cells from these assays to solid media to confirm growth. Here, we present a novel guide-frame system for accurate transfer of a 96-well assay to solid agar using a replicator.