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ARS Home » Plains Area » Lincoln, Nebraska » Agroecosystem Management Research » Research » Publications at this Location » Publication #425556
Research Project: Managing Manure as a Soil Resource for Improved Biosecurity, Nutrient Availability, and Soil Sustainability

Location: Agroecosystem Management Research

Title: ESBL Antibiotic Susceptibility Disc Diffusion Test SOP

Author
item Condon, Justine
item Durso, Lisa

Submitted to: Protocols.io
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/11/2025
Publication Date: 4/11/2025
Citation: Condon, J.C., Durso, L.M. 2025. ESBL Antibiotic Susceptibility Disc Diffusion Test SOP. Protocols.io. https://doi.org/10.17504/protocols.io.eq2lyxpqrgx9/v1.
DOI: https://doi.org/10.17504/protocols.io.eq2lyxpqrgx9/v1

Interpretive Summary: Some types of antibiotics are becoming less effective at curing diseases due to antibiotic resistance. One particularly dangerous type of resistance happens when an infectious disease causing bacteria makes a substance called Extended-spectrum ß-lactamase (ESBL). This protocol describes step-by-step instructions for how to perform a test that is used to determine if a particular bacteria can produce ESBL. It works by growing the bacteria, and then using antibiotic-infused discs to see which ones inhibit bacterial growth and by how much.

Technical Abstract: Disc (or Disk) diffusion by the Kirby-Bauer method is a standardized technique for testing rapidly growing pathogens. This procedure describes the steps for the ESBL antibiotic susceptibility disc diffusion test.