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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Plant Pathology Research » Research » Publications at this Location » Publication #425372
Research Project: Mitigation of Domestic, Exotic, and Emerging Diseases of Subtropical and Temperate Horticultural Crops

Location: Subtropical Plant Pathology Research

Title: Visual loop-mediated isothermal amplification (LAMP) assay for specific detection of groundnut ringspot and tomato spotted wilt orthotospoviruses

Author
item DE BREUIL, SOLEDAD - Instituto Nacional Tecnologia Agropecuaria
item Lopez Jr, Salvador
item DOTTORI, CAROLINA - Instituto Nacional Tecnologia Agropecuaria
item NOME, CLAUDIA - Instituto Nacional Tecnologia Agropecuaria
item Adkins, Scott

Submitted to: Journal of Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/30/2025
Publication Date: 10/20/2025
Citation: De Breuil, S., Lopez Jr, S., Dottori, C., Nome, C., Adkins, S.T. Highly sensitive reverse transcription loop-mediated isothermal amplification for detecting and identifying groundnut ringspot and tomato spotted wilt orthotospoviruses. Journal of Plant Pathology. 2025. https://doi.org/10.1007/s42161-025-02033-7.
DOI: https://doi.org/10.1007/s42161-025-02033-7

Interpretive Summary: Groundnut ringspot virus (GRSV) and tomato spotted wilt virus (TSWV) are significant pathogens of multiple agricultural crops worldwide. Accurate diagnostic tests are required to identify GRSV and TCSV, and allow rapid management decisions to be made. In this report, we describe development and validation of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for GRSV and TCSV for in-field diagnosis of these viruses. This approach to GRSV and TCSV detection will be useful to growers, Extension personnel, and local and Federal regulatory and research scientists.

Technical Abstract: Groundnut ringspot virus (GRSV) and tomato spotted wilt virus (TSWV) are highly destructive, thrips-transmitted orthotospoviruses that infect multiple agricultural and horticultural crops including peanuts, vegetables and ornamentals. It is important to reduce the risk of spreading these viruses by limiting movement of infected plant materials to other geographic areas by use of rapid, in-field diagnostic tests. Current laboratory assays for GRSV and TCSV require costly equipment, skilled personnel, and electricity. Here we report the development and validation of a simple reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay. This study highlights RT-LAMP as a rapid, species-specific, and highly sensitive tool for early detection of GRSV and TCSV in economically important crops, offering a promising solution for improved disease management.