Skip to main content
ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #425283
Research Project: Integration of Traditional Methods and Novel Molecular Strategies for Improving Disease Resistance and Input-use Efficiency in Peanut

Location: National Peanut Research Laboratory

Title: First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores,assembly and annotation of a new bacterial genome

Author
item Arias De Ares, Renee
item Dobbs, John
item Orner, Valerie
item CONFORTO, CINTHIA - Instituto Nacional Tecnologia Agropecuaria
item RAJO, ALENJANDRO - Instituto Nacional Tecnologia Agropecuaria
item CAZON, LUIS - Instituto Nacional De Tecnologia Agropecuaria
item Sobolev, Victor
item POWER, IMANA - Louisiana State University
item Lamb, Marshall
item Massa, Alicia

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/6/2025
Publication Date: 6/12/2025
Citation: Arias De Ares, R.S., Dobbs, J.T., Orner, V.A., Conforto, C., Rajo, A., Cazon, L.I., Sobolev, V., Power, I., Lamb, M.C., Massa, A.N. 2025. First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores,assembly and annotation of a new bacterial genome. Data in Brief. 61(111779). https://doi.org/10.1016/j.dib.2025.111779.
DOI: https://doi.org/10.1016/j.dib.2025.111779

Interpretive Summary: One possible method to evaluate presence of the peanut smut disease pathogen in soil is to quantify the number of teliospores of the pathogen. In this work, through sequencing, we found that teliospores could contain as little as 4% of Deoxyribonucleic acid(DNA) and Ribonucleic acid(RNA) of the pathogen, and the rest corresponded to various bacterial species. We identified those bacteria, assembled the complete genome of one of them (the most abundant), which seems to correspond to a new species, ensuing questions about its role in the pathosystem peanut/smut. Further research should consider the possible antagonistic effect of this bacterial species against the fungus.

Technical Abstract: These datasets correspond to sequencing of DNA and RNA extracted from surface-disinfected teliospores of the fungus Thecaphora frezzii Carranza and Lindquist, causal agent of smut disease in peanut (Arachis hypogaea L.). The DNA was sequenced using Pacific Biosciences (PacBio) Sequel II and RNA was sequenced in Illumina MiSeq 300 bp paired end (PE). Initial de novo assembly and Basic Local Alignment Search Tool (BLAST) of contigs to the 16S reference sequence database at NCBI had hits on seven bacterial species: Luteibacter pinisoli, Variovorax paradoxus, Rhizobium metallidurans, Caulobacter segnis, Roseateles violae, Novosphingobium rosa, Herbaspirillum seropedicae, and the fungus T. frezzii. High stringency mapping of the RNA sequences to the genomes of these organisms showed that 96% of the reads corresponded to bacteria and only 4% to T. frezzii. De novo assembly of PacBio reads revealed the whole genome of a Luteibacter sp. with 88.56% or lower similarity to those in NCBI database, whereas most RNA sequences (> 10 million reads) mapped to the new Luteibacter sp. genome assembled here. We annotated the new Luteibacter sp. genome, assessed its completeness by BUSCO, and aligned it to its closest relative, Luteibacter aegosomatissinici. We are not aware of any report that describes bacteria colonizing teliospores of T. frezzii. The DNA and RNA sequencing datasets provided here could be used to study the distribution of prokaryotes colonizing T. frezzii teliospores, and to explore the role of the microbiome of T. frezzii teliospores on the outcome of peanut smut disease.