Skip to main content
ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Mycology and Nematology Genetic Diversity and Biology Laboratory » Research » Publications at this Location » Publication #425012
Research Project: Plant-associated Nematode Management and Systematics and USDA Nematode Collection Curation

Location: Mycology and Nematology Genetic Diversity and Biology Laboratory

Title: ‘Candidatus Pseudomonas auctus’ sp. nov. JDE115 isolated from nodules on soybean (Glycines max)

Author
item ALI, MD SAHADAT - Virginia Tech
item MONY, FATIMA - Virginia Tech
item EVANS, MICHAEL - Virginia Tech
item RIDEOUT, STEVEN - Virginia Tech
item HAAK, DAVID - Virginia Tech
item Reis Vieira, Paulo
item EISENBACK, JONATHAN - Virginia Tech

Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/23/2025
Publication Date: 9/11/2025
Citation: Ali, M., Mony, F., Evans, M., Rideout, S., Haak, D., Reis Vieira, P.C., Eisenback, J.D. 2025. ‘Candidatus Pseudomonas auctus’ sp. nov. JDE115 isolated from nodules on soybean (Glycines max). PLOS ONE. 20(9). Article e0331920. https://doi.org/10.1371/journal.pone.0331920.
DOI: https://doi.org/10.1371/journal.pone.0331920

Interpretive Summary: Nematodes (microscopic roundworms) cause billions of dollars of crop losses every year. Many bacteria are known for their ability to produce chemicals that can control nematodes or enhance their plant hosts’ ability to resist infection. In this study, a newly identified bacterial species isolated from soybean roots in Virginia was described. Similar species of bacteria are known for their plant growth promoting and nematode control properties, and this new related bacterial species likely shares similar properties. Plant pathologists with the goal of enhancing soybean productivity will be able to use this bacterium and the chemicals it produces to develop new methods to control nematodes and enhance soybean growth, potentially saving soybean producers millions of dollars each year in crop losses.

Technical Abstract: A gram-negative, facultative anaerobic, rod-shaped, motile with peritrichous flagella, fluorescent bacterium, designated Pseudomonas auctus sp. nov. strain JDE115, was isolated from soybean root nodules in Virginia and characterized using a comprehensive integrative methodology. Growth of JDE115 occurred with 0–5.0% (w/v) NaCl (optimum 1%), at pH 6.0–10.0 (optimum pH 7.0), and at 10–40°C (optimum 28°C) in LB broth. Phylogenetic analyses based on the 16S rRNA gene placed the isolate as a member of a novel species within the genus Pseudomonas. Phylogenetic analyses based on whole-genome sequences showed JDE115 having the highest similarity to Pseudomonas glycinae MS586. Average Nucleotide Identity (ANI) analysis also revealed the highest similarity of JDE115 to Pseudomonas glycinae MS586 (94.59%), which is below the 95% threshold for species delineation. Genome-to-genome distance analysis showed a maximum value of 57.10% with the same strain, far below the 70% cutoff. The primary isoprenoid quinone detected in JDE115 was ubiquinone-9 (Q-9) and the DNA G+C content was 60.68%. Based on these phenotypic, chemotaxonomic, and phylogenetic data, strain JDE115 is proposed to represent a new species in the genus Pseudomonas, for which the name Pseudomonas auctus sp. nov. is proposed.