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ARS Home » Plains Area » Lincoln, Nebraska » Agroecosystem Management Research » Research » Publications at this Location » Publication #424471
Research Project: Managing Manure as a Soil Resource for Improved Biosecurity, Nutrient Availability, and Soil Sustainability

Location: Agroecosystem Management Research

Title: Standard uniplex PCR SOP for detection of Escherichia coli and Salmonella

Author
item Condon, Justine
item Durso, Lisa

Submitted to: Protocols.io
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2025
Publication Date: 3/28/2025
Citation: Condon, J.C., Durso, L.M. 2025. Standard uniplex PCR SOP for detection of Escherichia coli and Salmonella. Protocols.io. https://doi.org/10.17504/protocols.io.dm6gp9km1vzp/v1.
DOI: https://doi.org/10.17504/protocols.io.dm6gp9km1vzp/v1

Interpretive Summary: Escherichia coli and Salmonella are two foodborne pathogens that are transmitted via the fecal-oral route. One way to determine if a sample contains one of these organisms is to look for characteristic signatures in the bacterial DNA. The Polymerase Chain Reaction (PCR) is a specialized method that is able to detect even small numbers of the target cell. This protocol describes the procedures required to detect E. coli and Salmonella using standard PCR methods and detection via a process called gel electrophoresis.

Technical Abstract: Purpose To describe the correct procedures on how to set-up polymerase chain reaction (PCR) and run gel electrophoresis for the detection of Escherichia coli (E. coli) and Salmonella. Scope This protocol describes the procedures used to detect the presence of the invA gene from Salmonella and the uidA gene from E. coli using standard PCR, with detection via gel electrophoresis.