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ARS Home » Plains Area » Mandan, North Dakota » Northern Great Plains Research Laboratory » Research » Publications at this Location » Publication #424059
Research Project: Transdisciplinary Research that Improves the Productivity and Sustainability of Northern Great Plains Agroecosystems and the Well-Being of the Communities They Serve

Location: Northern Great Plains Research Laboratory

Title: Kinetics of Manganese Peroxidase Using Simple Phenolic Compounds as Substrates. Metabolites

Author
item GRUENBERG, MADELINE - Wright State University
item Halvorson, Jonathan
item SCHMIDT, MICHAEL - Wright State University

Submitted to: Metabolites
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/7/2025
Publication Date: 4/9/2025
Citation: Gruenberg, M., Halvorson, J.J., Schmidt, M.A. 2025. Kinetics of Manganese Peroxidase Using Simple Phenolic Compounds as Substrates. Metabolites. Metabolites. 15(4). Article 254. https://doi.org/10.3390/metabo15040254.
DOI: https://doi.org/10.3390/metabo15040254

Interpretive Summary: Plants produce a diverse class of compounds that range from small, simple molecules to large linked structures such as tannins. The smaller compounds such as phenolic acids can serve as substrates for soil microbes and enzymes. We monitored the activity of a redox active soil enzyme, manganese peroxidase (MnP), with three small phenols. The compounds used in this study were pyrogallol, gallic acid, and benzoic acid. Based on the kinetic parameters determined, pyrogallol and gallic acid are both substrates for MnP that react at different speeds and result in different products. Pyrogallol reacts faster and produces a more stable compound than gallic acid. Benzoic acid is not a substrate for MnP.

Technical Abstract: Secondary metabolites are a diverse class of compounds that include small phenols to large polymeric structures such as tannins. The smaller compounds such as phenolic acids can serve as substrates for soil microbes and enzymes. We monitored the activity of a redox active soil enzyme, manganese peroxidase (MnP), with three small phenols. The compounds used in this study were pyrogallol, gallic acid, and benzoic acid. Based on the kinetic parameters determined, pyrogallol and gallic acid are both substrates for MnP with different products and kinetics. Pyrogallol reacts faster and produces a more stable quinone than gallic acid. Benzoic acid is not a substrate for MnP.