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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Publications at this Location » Publication #423752
Research Project: Enhancing Yield, Disease Resistance, and Agronomic Performance in Edible Legumes

Location: Grain Legume Genetics Physiology Research

Title: An RNase H-Like gene complements resistance to Bean common mosaic necrosis virus in Phaseolus vulgaris

Author
item SOLER-GARZON, ALVARO - Washington State University Extension Service
item Miklas, Phillip

Submitted to: The Plant Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/4/2025
Publication Date: 5/19/2025
Citation: Soler-Garzon, A., Miklas, P.N. 2025. An RNase H-Like gene complements resistance to Bean common mosaic necrosis virus in Phaseolus vulgaris. The Plant Genome. 18(2). e70046. https://doi.org/10.1002/tpg2.70046.
DOI: https://doi.org/10.1002/tpg2.70046

Interpretive Summary: Bean common mosaic and necrosis viruses (BCMV/BCMNV) plague common bean production worldwide. Genetic resistance provides the best control of this viral disease. Many genes interact to confer resistance. Herein we describe a new candidate gene RNase H-Like 1 which enhances the resistance effect of bc-1 gene. The bc-1 is a major resistance gene used by dry bean breeders in the U.S. and elsewhere to control this disease. The discovery of this additional RNase gene which strengthens the resistance effect of bc-1 will be an important weapon in the war chest for combatting this devasting pathogen going forward.

Technical Abstract: Genetic resistance is critical for controlling potyvirus diseases in common bean. Three uncharacterized RNase H-Like 1, 2, and 3 genes were identified near the bc-1 resistance gene locus in P. vulgaris through a BLAST search against an RNase H family member associated with the Rsv4 locus in soybean. The synteny between bc-1 and Rsv4 gene regions in P. vulgaris and G. max was previously established and is further strengthened by the presence of orthologous RNase H-Like genes within the same region in both species. Two independent studies identified separate candidate genes for Rsv4 in G. max, an RNase H-Like gene with 3.6 kb deletion and then a malectin-like receptor kinase GmMLRK1 (haplotype 1). GmMLRK1 had a major resistance effect against SMV that was enhanced slightly by the presence of RNase H-like resistance gene. For P. vulgaris we observed a similar reaction. A receptor-like kinase (PvRLK) was identified as the candidate gene for bc-1, and in this study, we identified an RNase H-Like 1 gene which enhanced the resistance effect of bc-1. The present study advances the understanding of the complex mechanisms underlying BCMNV resistance in common bean. We demonstrate the complementarity of RNase H-Like 1 with I, bc-1, and bc-u genes, and propose a new SNP marker G03_4166082 to facilitate the introgression and tracking of RNase H-Like 1 gene in breeding lines for enhanced resistance to BCMNV. Further research on the potential complementary effect of RNase H-Like 1 with other resistance genes and against different BCMV and BCMNV strains representing different pathogroups is warranted.