Seed biology and regeneration niche of the threatened cold desert perennial Ivesia webberi A. Gray

Authors: BOROKINI, ISRAEL - Montana State University; FRANCE, MICHAEL - Montana State University; Harmon, Daniel; SHOEMAKER, KEVIN - University Of Nevada; WEISBERG, PETER - University Of Nevada; PEACOCK, MARY - University Of Nevada

Submitted to: Plant Diversity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/11/2025
Publication Date: 5/5/2025
Citation: Borokini, I.T., France, M., Harmon, D.N., Shoemaker, K., Weisberg, P., Peacock, M. 2025. Seed biology and regeneration niche of the threatened cold desert perennial Ivesia webberi A. Gray. Plant Diversity. 16. Article 1568951. https://doi.org/10.3389/fpls.2025.1568951.
DOI: https://doi.org/10.3389/fpls.2025.1568951

Interpretive Summary: Understanding the regeneration niche is of critical importance for the conservation of rare plants, yet species-specific information is often lacking for key components of the plant life cycle such as seed dormancy and germination. We conducted a detailed study of the regeneration niche for Ivesia webberi, a U.S. federally threatened forb that is endemic to the Great Basin Desert. Using seeds collected from 11 populations across a span of years, we investigated seed storage behavior, embryo morphology, and interannual and interpopulation seed viability, while testing the efficacy of alternative nondestructive methods to assess seed viability. We also studied the effects of various pre-incubation and incubation treatments on germination rates, speed, and synchrony. An examination of x-ray images showed that I. webberi have non-endospermic seeds with spatulate embryos. We observed a significant reduction in seed viability over three years, suggesting a recalcitrant storage behavior. Seed viability exhibited significant interannual, but not interpopulation, variation across 11 I. webberi populations. Both the x-ray and multispectral imaging are promising nondestructive methods that can replace the widely used, but destructive, tetrazolium test. Across all 68 germination treatments, seed germination was higher, faster, and more synchronized under warmer cold- stratified incubation temperatures. Seed germination was significantly increased by pre- incubation chilling and reduced by pre-incubation heat treatments, while pre- incubation and incubation light exposures had no effect. Both the seed embryo morphology and germination experiments suggest physiological dormancy in I. webberi. Results suggest that warmer and shorter winter, such as are consistent with predicted climate change, could increase germination but also lead to shifts in regeneration phenology that increase vulnerability of seedlings to frost.

Technical Abstract: Understanding the regeneration niche is of critical importance for the conservation of rare plants, yet species-specific information is often lacking for key components of the plant life cycle such as seed dormancy and germination. Using seeds collected from 11 populations of Ivesia webberi over multiple years, we investigated seed storage behavior, embryo morphology, and inter-annual and inter-population seed viability, while testing the efficacy of alternative nondestructive methods to assess seed viability. Seed germination was significantly increased by pre- incubation chilling and reduced by pre-incubation heat treatments, while pre- incubation and incubation light exposures had no effect. Both the seed embryo morphology and germination experiments suggest physiological dormancy in Ivesia webberi. Results suggest that warmer and shorter winterS, such as are consistent with predicted climate change, could increase germination but also lead to shifts in regeneration phenology that can also increase vulnerability of seedlings to frost.