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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #422375
Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Genetic and physical localization of Hessian fly resistance genes in barley

Author
item KARKI, MANILA - North Dakota State University
item ROBBANI, MD GOLAM - North Dakota State University
item Nandety, Raja Sekhar
item Fiedler, Jason
item LIU, ZHAOHUI - North Dakota State University
item Yang, Shengming

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2024
Publication Date: 1/3/2025
Citation: Karki, M., Robbani, M., Nandety, R.S., Fiedler, J.D., Liu, Z., Yang, S. 2025. Genetic and physical localization of Hessian fly resistance genes in barley. Plant & Animal Genome Conference; San Diego, California. Poster No. P472.

Interpretive Summary:

Technical Abstract: Hessian fly, (Mayetiola destructor) is one of the most significant insects worldwide that attack cereals including wheat, barley, and rye. Interestingly, the outcome of interaction between Hessian fly and its host is controlled by the gene-for-gene model. Hessian fly resistance has been extensively searched in wheat, and at least 37 resistance loci (H1-H36 plus Hdic) have been reported. Although Hessian fly is a significant pest of barley, so far there were no attempts towards identification of HF resistance as well as their potential DNA markers in barley. In the present study, genome-wide association study was performed in a core collection of 240 lines to identify barley resistance to Hessian fly. Of those, 210 lines were susceptible, indicating an urgent need to enhance barley resistance to Hessian fly. A total of 5 resistance loci was identified on 1H, 3H, 4H, 5H, and 6H, respectively. The locus on 4H (named HvRHF1 hereafter) showed a significant effect on disease phenotype, and it was selected for fine mapping using a biparental population. Assisted with a total of 2400 F2 plants, the HvRHF1 gene was delimited to a physical interval spanning ~400 kb region containing six NBS-LRR genes, characteristic of resistance genes. These candidate genes will be tested in a susceptible variety Golden promise to verify their function in Hessian fly resistance. Therefore, our study provided resistance resource and tools for breeding programs, and it also offered a genetic map and candidate genes for cloning of the HvRHF1 gene.