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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #422231
Research Project: Genetic Resource and Information Management for Pulse, Temperate Forage Legume, Oilseed, Vegetable, Grasses, Sugar, Ornamental, and Other Crops

Location: Plant Germplasm Introduction and Testing Research

Title: Genome-wide association study and genomic selection in lentils (lens culinaris) for seed protein concentration

Author
item UHDRE, RENAN - Washington State University
item COYNE, CLARICE - Washington State University
item MA, YU - The Ohio State University
item Bourland, Britton
item SARI, HATICE - Washington State University
item FERREIRA, SUNGIL - University Of Arkansas
item Warburton, Marilyn

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/18/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary: NA

Technical Abstract: Plant-based protein from lentils is increasingly popular as a healthy and environmentally friendly alternative to animal-based protein. Lentils offer a promising protein source due to their non-GMO nature, low allergenicity, and rich nutritional composition. However, seed protein concentration varies widely among lentil germplasm, necessitating enhancements in protein levels for commercial varieties to meet the growing demand for plant protein. This study investigated the natural variation in seed protein concentration across a USDA lentil diversity panel, identifying candidate genes involved in protein regulation. We phenotyped 183 accessions in a randomized complete block design (RCBD) with four replicates over three years, assessing seed protein concentration using a LECO nitrogen analyzer. Protein concentration was calculated based on nitrogen content. Best Linear Unbiased Estimates (BLUEs) were calculated and used in a genome-wide association study (GWAS) using 1.7 million high-quality single nucleotide polymorphism (SNP) markers. This analysis identified significant variants and neighboring candidate genes associated with seed protein concentration. To further leverage this genomic information, a genomic selection approach will be implemented, integrating phenotypic data with genome-wide markers to predict breeding values. To date, no study has reported the potential of GS for enhancing protein concentration in lentil, although GS results have been reported for other important traits. We will use a second genotyped international diversity panel (AGILE) to validate GS models and provide valuable insights to breeders working to enhance protein concentration and facilitate crop improvement in lentil.