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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #421953

Research Project: Intervention Strategies to Control Endemic and New Emerging and Re-Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Single-plex brightfield detection of CD3e, IBA-1, Pax5, Ki-67, and pan-cytokeratin type I/II in formalin-fixed, paraffin-embedded tonsil tissues of pigs, cattle, and white-tailed deer

Author
item Wiarda, Jayne
item ZANELLA, ERALDO - Oak Ridge Institute For Science And Education (ORISE)
item Loving, Crystal
item Palmer, Mitchell

Submitted to: Protocols.io
Publication Type: Other
Publication Acceptance Date: 12/19/2024
Publication Date: 12/19/2024
Citation: Wiarda, J.E., Zanella, E.L., Loving, C.L., Palmer, M.V. 2024. Single-plex brightfield detection of CD3e, IBA-1, Pax5, Ki-67, and pan-cytokeratin type I/II in formalin-fixed, paraffin-embedded tonsil tissues of pigs, cattle, and white-tailed deer . Protocols.io. https://doi.org/10.17504/protocols.io.14egn9zrpl5d/v1.
DOI: https://doi.org/10.17504/protocols.io.14egn9zrpl5d/v1

Interpretive Summary:

Technical Abstract: Detection of CD3e (T cells), IBA-1 (macrophages/dendritic cells), Pax5 (B cells), Ki-67 (proliferating cells), or pan-cytokeratin type I/II (epithelial cells) in formalin-fixed, paraffin-embedded (FFPE) palatine tonsil tissues from pigs, cattle, and white-tailed deer.