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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #421943
Research Project: Intervention Strategies to Control Endemic and New Emerging and Re-Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Co-detection of RNA and protein (CD3e, IBA-1, Pax5, Ki-67, or pan-cytokeratin type I/II) in formalin-fixed, paraffin-embedded tonsil tissues of pigs, cattle, and white-tailed deer

Author
item Wiarda, Jayne
item Shircliff, Adrienne
item ZANELLA, ERALDO - Oak Ridge Institute For Science And Education (ORISE)
item Loving, Crystal
item Palmer, Mitchell

Submitted to: Protocols.io
Publication Type: Other
Publication Acceptance Date: 12/19/2024
Publication Date: 12/19/2024
Citation: Wiarda, J.E., Shircliff, A.L., Zanella, E.L., Loving, C.L., Palmer, M.V. 2024. Co-detection of RNA and protein (CD3e, IBA-1, Pax5, Ki-67, or pan-cytokeratin type I/II) in formalin-fixed, paraffin-embedded tonsil tissues of pigs, cattle, and white-tailed deer. Protocols.io. https://doi.org/10.17504/protocols.io.n92ldr5o8g5b/v1.
DOI: https://doi.org/10.17504/protocols.io.n92ldr5o8g5b/v1

Interpretive Summary:

Technical Abstract: Detection of CD3' (T cells), IBA-1 (macrophages/dendritic cells), Pax5 (B cells), Ki-67 (proliferating cells), or pan-cytokeratin type I/II (epithelial cells) in conjunction with an RNA marker (housekeeping gene, UBC, used herein) in formalin-fixed, paraffin-embedded (FFPE) palatine tonsil tissues from pigs, cattle, and white-tailed deer.