Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #421918
Research Project: Intervention Strategies to Control Endemic and New Emerging and Re-Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Multiplexed brightfield detection of CD3e, IBA-1, and Pax5 in formalin-fixed, paraffin-embedded lymph node tissues of pigs, cattle, and white-tailed deer

Author
item Wiarda, Jayne
item ZANELLA, ERALDO - Oak Ridge Institute For Science And Education (ORISE)
item Palmer, Mitchell

Submitted to: Protocols.io
Publication Type: Other
Publication Acceptance Date: 12/19/2024
Publication Date: 12/19/2024
Citation: Wiarda, J.E., Zanella, E.L., Palmer, M.V. 2024. Multiplexed brightfield detection of CD3e, IBA-1, and Pax5 in formalin-fixed, paraffin-embedded lymph node tissues of pigs, cattle, and white-tailed deer . Protocols.io. https://doi.org/10.17504/protocols.io.rm7vzk394vx1/v1.
DOI: https://doi.org/10.17504/protocols.io.rm7vzk394vx1/v1

Interpretive Summary:

Technical Abstract: Simultaneous detection of CD3e (T cells; stained in brown), IBA-1 (macrophages/dendritic cells; stained in blue), and Pax5 (B cells; stained in red) in formalin-fixed, paraffin-embedded (FFPE) tracheobronchial lymph node tissues from pigs, cattle, and white-tailed deer.