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ARS Home » Pacific West Area » Logan, Utah » Forage and Range Research » Research » Publications at this Location » Publication #421811

Research Project: Improved Plant Genetic Resources and Methods to ensure Resilient and Productive Rangelands, Pastures, and Turf Landscapes

Location: Forage and Range Research

Title: The Construction of Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species

Author
item WANG, LIN - Shandong Agricultural University
item LIANG, SHUANG - Shandong Agricultural University
item QI, FEI - Shandong Agricultural University
item BAO, YINGUANG - Shandong Agricultural University
item Wang, Richard
item LI, XINGFENG - Shandong Agricultural University

Submitted to: Plants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/10/2025
Publication Date: 1/12/2025
Citation: Wang, L., Liang, S., Qi, F., Bao, Y., Wang, R., Li, X. 2025. Construction of Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species. Plants. 2025 14(2)196. https://www.mdpi.com/2223-7747/14/2/196.
DOI: https://doi.org/10.3390/plants14020196

Interpretive Summary: The genome composition of intermediate wheatgrass (IWG; Thinopyrum intermedium (Host) Barkworth & D.R. Dewey; 2n = 6x = 42), is complex and continued to be a subject of investigation. In this study, fluorescence in situ hybridization (FISH) was used to analyze the karyotype of Th. intermedium and its related species. Using St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum as probes, the chromosomes of Th. intermedium were classified by FISH, denoted as JvsJvsJrJrStSt. FISH karyotype was established using pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4 and pAs1-6 as a combined multiplex oligonucleotide probe. MATO software was used to analyze chromosome length, arm ratio and karyotype structure. Based on results of FISH, standard karyotypes of Th. intermedium and its potential progenitor species were constructed. These standard karyotypes revealed that there was evolutionary parallelism between genome and karyotype, but due to the complexity of evolution, the FISH signal of Th. intermedium was abundant and asymmetrical.

Technical Abstract: The genome composition of intermediate wheatgrass (IWG; Thinopyrum intermedium (Host) Barkworth & D.R. Dewey; 2n = 6x = 42), is complex and continued to be a subject of investigation. In this study, fluorescence in situ hybridization (FISH) was used to analyze the karyotype of Th. intermedium and its related species. Using St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum as probes, the chromosomes of Th. intermedium were classified by FISH, denoted as JvsJvsJrJrStSt. FISH karyotype was established using pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4 and pAs1-6 as a combined multiplex oligonucleotide probe. MATO software was used to analyze chromosome length, arm ratio and karyotype structure. The karyotype formula of Th. intermedium is K(2n) = 6X=42=36m+6sm, and that of Th. junceiforme is K(2n) =4X=28=22m+6sm. The karyotype formula of Th. elongatum and Th. bessarabicum is K(2n) =2X=14=12m+ 2sm, of Ps. spicata is K(2n) =2X=14=2M+12m, and of Da. villosum was K(2n) =2X=14=12m+2sm. Based on results of FISH, standard karyotypes of Th. intermedium and its potential progenitor species were constructed. These standard karyotypes revealed that there was evolutionary parallelism between genome and karyotype, but due to the complexity of evolution, the FISH signal of Th. intermedium was abundant and asymmetrical.