Location: Forage and Range Research
Title: The Construction of Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor SpeciesAuthor
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WANG, LIN - Shandong Agricultural University |
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LIANG, SHUANG - Shandong Agricultural University |
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QI, FEI - Shandong Agricultural University |
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BAO, YINGUANG - Shandong Agricultural University |
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Wang, Richard |
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LI, XINGFENG - Shandong Agricultural University |
Submitted to: Plants
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 1/10/2025 Publication Date: 1/12/2025 Citation: Wang, L., Liang, S., Qi, F., Bao, Y., Wang, R., Li, X. 2025. Construction of Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species. Plants. 2025 14(2)196. https://www.mdpi.com/2223-7747/14/2/196. DOI: https://doi.org/10.3390/plants14020196 Interpretive Summary: The genome composition of intermediate wheatgrass (IWG; Thinopyrum intermedium (Host) Barkworth & D.R. Dewey; 2n = 6x = 42), is complex and continued to be a subject of investigation. In this study, fluorescence in situ hybridization (FISH) was used to analyze the karyotype of Th. intermedium and its related species. Using St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum as probes, the chromosomes of Th. intermedium were classified by FISH, denoted as JvsJvsJrJrStSt. FISH karyotype was established using pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4 and pAs1-6 as a combined multiplex oligonucleotide probe. MATO software was used to analyze chromosome length, arm ratio and karyotype structure. Based on results of FISH, standard karyotypes of Th. intermedium and its potential progenitor species were constructed. These standard karyotypes revealed that there was evolutionary parallelism between genome and karyotype, but due to the complexity of evolution, the FISH signal of Th. intermedium was abundant and asymmetrical. Technical Abstract: The genome composition of intermediate wheatgrass (IWG; Thinopyrum intermedium (Host) Barkworth & D.R. Dewey; 2n = 6x = 42), is complex and continued to be a subject of investigation. In this study, fluorescence in situ hybridization (FISH) was used to analyze the karyotype of Th. intermedium and its related species. Using St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum as probes, the chromosomes of Th. intermedium were classified by FISH, denoted as JvsJvsJrJrStSt. FISH karyotype was established using pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4 and pAs1-6 as a combined multiplex oligonucleotide probe. MATO software was used to analyze chromosome length, arm ratio and karyotype structure. The karyotype formula of Th. intermedium is K(2n) = 6X=42=36m+6sm, and that of Th. junceiforme is K(2n) =4X=28=22m+6sm. The karyotype formula of Th. elongatum and Th. bessarabicum is K(2n) =2X=14=12m+ 2sm, of Ps. spicata is K(2n) =2X=14=2M+12m, and of Da. villosum was K(2n) =2X=14=12m+2sm. Based on results of FISH, standard karyotypes of Th. intermedium and its potential progenitor species were constructed. These standard karyotypes revealed that there was evolutionary parallelism between genome and karyotype, but due to the complexity of evolution, the FISH signal of Th. intermedium was abundant and asymmetrical. |