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ARS Home » Plains Area » Lincoln, Nebraska » Agroecosystem Management Research » Research » Publications at this Location » Publication #421681
Research Project: Managing Manure as a Soil Resource for Improved Biosecurity, Nutrient Availability, and Soil Sustainability

Location: Agroecosystem Management Research

Title: 4-Gene standard uniplex PCR protocol for antibiotic resistance genes and determinants sul1, erm(B), ctx-m32, and intI1

Author
item Condon, Justine
item Durso, Lisa

Submitted to: Protocols.io
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/13/2024
Publication Date: 12/13/2024
Citation: Condon, J.C., Durso, L.M. 2024. 4-Gene standard uniplex PCR protocol for antibiotic resistance genes and determinants sul1, erm(B), ctx-m32, and intI1. Protocols.io. https://doi.org/10.17504/protocols.io.yxmvme6jbg3p/v1.
DOI: https://doi.org/10.17504/protocols.io.yxmvme6jbg3p/v1

Interpretive Summary: While there is continued concern over antibiotic resistance originating from agriculture and the environment, it remains very difficult to measure. This protocol provides a curated set of four genes designed to provide actionable information on antibiotic resistance in complex agricultural and environmental samples. It includes antibiotic resistance genes from three commonly assayed drug classes (sul1, erm(b) and ctx-m) and one determinant often associated with human-impacted samples (the integrase gene intI1). This protocol describes the correct procedures on how to set-up, run, and record results for PCR assays (polymerase chain reaction) used to measure these targets in feces, soil, water, and other environmental samples.

Technical Abstract: A suite of four antibiotic resistance determinants were selected as a general screening tool for agricultural and environmental samples. It included antibiotic resistance genes from three commonly assayed drug classes (sul1, erm(b) and ctx-m) and one determinant often associated with human-impacted samples (the integrase gene intI1). This protocol describes the correct procedures on how to set-up, run, and record results for PCR assays.