Effect of synbiotic supplementation on cecal microbiota during exposure to subclinical doses of fumonisin and deoxynivalenol in broiler chickens

Authors: DASIREDDY, JOSEPH RISHITHA - University Of Georgia; KAPPARI, LAHARIKA - University Of Georgia; PENDER, CHASITY - Dsm; DOUPOVEC, BARBARA - Dsm; MURUGESAN, RAJ - Dsm; APPLEGATE, TODD - University Of Georgia; Shanmugasundaram, Revathi

Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: 11/27/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary: N/A

Technical Abstract: Synbiotics modulate the cecal microbiota in chickens, favoring the growth of beneficial bacteria to mitigate the effect of mycotoxins such as fumonisins (FUM) and deoxynivalenol (DON) through biotransformation. This study aimed to identify the effect of supplementation of synbiotic at 0.05% (PoultryStar BRO, dsm-firmenich) on the cecal microbiota composition, diversity, and short-chain fatty acid (SCFA) profile in broilers exposed to subclinical concentrations of FUM and DON. A total of 360 one-day-old broilers were distributed into 4 dietary treatments: T1(Control), T2 (Synbiotic at 0.05%), T3 (8.5 FUM+ 3.8 DON), T4 (8.6 FUM+ 3.9 DON + Synbiotic at 0.05%) mg/kg diet. Cecal contents were collected on day 35, and bacterial compositions were analyzed using Illumina MiSeq sequencing of the V3–V4 region of the 16S rRNA gene. Relative abundance of families and alpha diversity indices were analyzed using the Kruskal-Wallis H Test and SCFA was analyzed using one-way ANOVA. The results showed that on day 35, the acetate levels increased in T2, T3, and T4 by 5.92%, 6.83%, and 5.18% respectively compared to that of the control(T1) group. Total SCFAs increased by 7.07% and 3.56% in T2 & T3 but decreased by 0.78% in T4 compared to the control group. (p > 0.1). At family level, the relative abundance fold change for Bacillaceae increased by 3.47 and 1.03 in T2 and T4 groups respectively whereas decreased by 0.6 in T3, Bacteriodaceae increased by 1.19 in T2 whereas decreased by 0.86 and 0.67 in T3 & T4 groups compared to the control group (p < 0.05). The fold change of relative abundance of Enterobacteriaceae increased by 1.28 in T2 whereas decreased by 0.9, 0.64 in T3 & T4 groups respectively (p < 0.05). Lactobacillaceae was increased by 1.45, 2.66 and 2.52 in T2, T3& T4 groups respectively(p < 0.05). There were no significant differences in the abundance of Lachnospiraceae, Ruminococcaceae, and alpha diversity indices (Shannon, chao1, faith_PD) in treatment groups compared to that of the control group (p > 0.05). In conclusion, synbiotic supplementation at 0.05% had a positive impact on the cecal microbiota by increasing the beneficial bacteria and modulating SCFAs concentration. Keywords: Mycotoxin, Broilers, Synbiotics, microbiota