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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #420900
Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Genetic analysis of adult plant resistance for oat crown rust in a nested association mapping population

Author
item NLN, AMANDEEP - North Dakota State University
item Sapkota, Suraj
item Esvelt Klos, Kathy
item Yimer, Belayneh
item Fiedler, Jason
item Nandety, Raja Sekhar
item HARRISON, STEPHEN - Louisiana State University
item BABAR, ALI - University Of Florida
item MCMULLEN, MICHAEL - North Dakota State University
item Carlson, Craig

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2024
Publication Date: 12/1/2024
Citation: Nln, A., Sapkota, S., Esvelt Klos, K.L., Yimer, B.A., Fiedler, J.D., Nandety, R., Harrison, S., Babar, A., Mcmullen, M., Carlson, C.H. 2024. Genetic analysis of adult plant resistance for oat crown rust in a nested association mapping population. Meeting Abstract. Poster No. PE58093.

Interpretive Summary:

Technical Abstract: Oat crown rust is caused by the fungal pathogen, Puccinia coronata f. sp. avenae (Pca), that can cause severe damage in all regions where oat is cultivated. As a highly volatile and genetically diverse pathogen, the identification of new genomic regions associated with quantitative, adult plant resistance (APR) will reinforce oat breeding efforts. Here, we use linkage and association mapping to identify genetic loci associated with crown rust resistance in a nested association mapping population, dubbed “HXS”. Donor lines (CIav 2272, PI 140903, PI 237090, PI 287296, PI 285583, and PI 219765) identified among oat accessions in the National Small Grains Collection (USDA-ARS, Aberdeen, ID). Each donor line was crossed with the spring oat cultivar ‘Idahoat’, yielding 612 recombinant inbred lines (RILs). The HXS was genotyped via genotyping-by-sequencing and evaluated for APR for crown rust under field condition in 2024 in Fargo, ND, using a modified Cobb scale. Phenotypic evaluation identified 93 RILs exhibiting moderate to good APR for crown rust, and importantly, two RILs derived from the same donor parent had no signs or symptoms of disease. Association mapping was performed to identify variants and genes associated with APR, yielding new loci and theories on the genomic basis of APR. RILs expressing APR identified in the study will be intercrossed and crossed with elite lines to develop stable crown rust resistant cultivars.