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ARS Home » Southeast Area » Auburn, Alabama » Soil Dynamics Research » Research » Publications at this Location » Publication #420237
Research Project: Agronomic and Engineering Solutions for Conventional and Organic Conservation Agricultural Systems in the Southeastern U.S.

Location: Soil Dynamics Research

Title: Development and analysis of infectious and fluorescent clones of cotton leafroll dwarf virus- AL

Author
item CLARK, WILSON - Auburn University
item LIVINGSTON, RACHEL - Auburn University
item HIRSCH, KAYLEE - Auburn University
item KOEBERNICK, JENNY - Auburn University
item KANG, S - Auburn University
item Price, Andrew
item CONNER, KASSIE - Auburn University
item JACOBSON, ALANA - Auburn University
item MARTIN, KATE - Auburn University

Submitted to: PhytoFrontiers
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/6/2025
Publication Date: N/A
Citation: N/A

Interpretive Summary: Cotton leafroll dwarf virus (CLRDV) is a plant pathogen that was first detected in the United States in 2017 and has caused yield loss in cotton (Gossypium hirsutum L) production. In Argentina, infectious clones of both the typical and atypical strains of CLRDV were developed to further research into the virus. In this study two infectious clones based on the original Alabama isolate were developed to help further research into this emerging pathogen. This clone produces EGFP and is visible by fluorescence microscopy in infiltrated cotton cotyledons and can be detected with commercial antibodies. This study presents the first infectious clones of CLRDV in the United States and adds to the growing knowledge of Poleroviruses.

Technical Abstract: Cotton leafroll dwarf virus (CLRDV) is a plant pathogen that was first detected in the United States in 2017 and has caused yield loss in cotton (Gossypium hirsutum L) production. In Argentina, infectious clones of both the typical and atypical strains of CLRDV were developed to further research into the virus. In this study two infectious clones based on the original Alabama isolate were developed to help further research into this emerging pathogen. Both are Agrobacterium tumefaciens expression constructs which are agroinfiltrated into plants to cause infection. CLRDV-Macon1 (abbreviated CLRDV-Ma1) was created by inserting the full sequence of CLRDV into pJL89, an agrobacterium expression vector. This virus clone systemically infects both cotton and Nicotiana benthamiana and is detectable by PCR assays. CLRDV-M-GFP was created by replacing a C-terminal section of ORF3-5 from CLRDV-Ma1 with EGFP. This clone produces EGFP and is visible by fluorescence microscopy in infiltrated cotton cotyledons and can be detected with commercial antibodies. This study presents the first infectious clones of CLRDV in the United States and adds to the growing knowledge of Poleroviruses.