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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Molecular Plant Pathology Laboratory » Research » Publications at this Location » Publication #420152
Research Project: Developing Biotechnologies to Improve Pest and Pathogen Resistance, Yield, and Quality in Sugarbeet

Location: Molecular Plant Pathology Laboratory

Title: Transcriptome sample statistics for the sugar beet root maggot (Tetanops myopaeformis) infecting sugar beet

Author
item ACHARYA, SUDHA - Towson University
item ALKHAROUF, NADIM - Towson University
item TEHSEEN, MUHAMMAD - North Dakota State University
item Chu, Chenggen
item Klink, Vincent

Submitted to: Bioinformation
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/31/2024
Publication Date: 12/31/2024
Citation: Acharya, S., Alkharouf, N.W., Tehseen, M.T., Chu, C.N., Klink, V.P. 2024. Transcriptome sample statistics for the sugar beet root maggot (Tetanops myopaeformis) infecting sugar beet. Bioinformation. 20(12):1881-1885. https://doi.org/10.6026/9732063002001881.
DOI: https://doi.org/10.6026/9732063002001881

Interpretive Summary: Sugar beet is valued at $1 B in the U.S., and $4.6 B, globally. The sugar beet root maggot (SBRM) is the most devastating sugar beet pathogen in North America, with the potential of causing 100% crop loss, locally and is spreading throughout the sugar beet growing range at an accelerated rate. Therefore, finding solutions to control SBRM is of urgent need. Gene expression studies of the SBRM larvae infecting two different resistant and susceptible varieties was performed with the aid of the newly sequenced and annotated SBRM genome. The analysis identified genes that are expressed specifically during the susceptible or resistant reactions. The work has identified genes that are targets for SBRM control, management, and genetic interference with the goal of aiding stakeholders to eliminate this pest.

Technical Abstract: The sugar beet root maggot (SBRM), Tetanops myopaeformis (von Röder) insect pathogen devastates sugar beet (SB), Beta vulgaris ssp, vulgaris (B. vulgaris), one of only two plants from which significant global raw sugar is produced, $1B, U.S., $4.6 B, globally. Larval SBRMs experiencing F1010 and L19 susceptible or F1016 and F1024 resistant SB responses are RNA sequenced, sampled at time = 0 hours post infection [hpi], 24, 48 and 72 hpi. Transcriptomic analyses determined the number of reads per sample, mapped the transcripts to the recently sequenced SBRM TmSBRM_v1.0 draft genome, and identified genes that relate to the resistant and susceptible responses. The RNA-seq study provides data for generating differential expression analyses, yielding an understanding SBRM biology, control strategy development, relationship to model and non-model organisms, and aiding sugar beet improvement for stakeholders.