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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food Processing and Sensory Quality Research » Research » Publications at this Location » Publication #419811
Research Project: Reducing the Development and Severity of Allergy to Peanuts and Tree Nuts

Location: Food Processing and Sensory Quality Research

Title: Phenylglyoxal modification of purified Ana o 3: characterizing changes in protein solubility, structure, and antibody recognition

Author
item Brown, Chaka
item Vuong, Tien
item PAYNE, ALEXIS - Oak Ridge Institute For Science And Education (ORISE)
item Dupre, Rebecca
item Boue, Stephen
item Smith, Brennan
item Mattison, Christopher

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary: N/A

Technical Abstract: Ana o 3 is a potent cashew nut allergen, and recognition of Ana o 3 by immunoglobulin IgE antibodies is a good indication of clinically severe cashew nut allergy. Phenylglyoxal (PG) is an aromatic a-dicarbonyl compound that can specifically modify arginine residues in proteins. Purified Ana o 3 was treated with PG, modification sites were identified, and changes in solubility, protein structure, and antibody recognition were evaluated. Mass-spectrometric analysis identified numerous Ana o 3 arginine residues including R48, R50, R74, R85, R86, and R111 that were modified by PG treatment. Immunoblot assay with a battery of polyclonal and monoclonal anti-Ana o 3 antibodies indicated PG treatment led to reduced antibody binding. Exposure to PG also caused a shift toward larger Ana o 3 particle sizes, decreased zeta potential, and ninhydrin assay confirmed a decreased abundance of primary amines relative to untreated control samples. Additional chemical and protein structure assays indicated changes in Ana o 3 arginine content and Ana o 3 conformation. Overall, the results indicated PG modified several arginine residues within Ana o 3 and these modifications result in altered size, charge, structure, and antibody recognition of Ana o 3.