Location: Food Animal Metabolism Research
Title: Evaluation of PFAS cytotoxicity towards human gastric cells with or without the presence of microplasticsAuthor
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Shelver, Weilin |
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Billey, Lloyd |
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McGarvey, Amy |
Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only Publication Acceptance Date: 12/10/2024 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Per- and polyfluoroalkyl substances (PFAS) and micro/nanoplastics (MP/NP) are ubiquitous environmental contaminants. Both pollutants have been detected in food matrices used for human consumption. Although limited data exist for MP/NP cytotoxicity towards gastric cells, the cytotoxic effects of combinations of PFAS and MP/NP towards human gastric cells, SNU-1, have not been explored. In this study, the alamarBlue® assay was used to evaluate the cytotoxicity of C8- (PFOA, PFOS), C9- (PFNA, PFNS) and C10- (PFDA, and PFDS) PFAS with, or without, the presence of aminated, carboxylated, and non-modified polystyrene particles (50, 200, and 1000 nm) during 1-, 2-, and 4-hour incubations. At one hour, the relative cytotoxicity was PFDA > PFOS > PFNS > PFNA > PFDS > PFOA indicating chain length as well as the PFAS “head” contributed to cytotoxicity. Cytotoxicity to sulfonic acids was C8 > C9 > C10 while carboxylic acids exhibited a reverse trend. Increasing the incubation time from 1 to 4 hours further increased cytotoxicity by 7 – 31%. When PFAS was co-cultured with MP/NP the cytotoxicity to SNU-1 cells increased. Smaller sized particles co-cultured with PFAS induced greater cytotoxicity in comparison to larger sized particles. Aminated beads co-cultured with PFAS had the greatest cytotoxicity in comparison to the other surface functional groups. In summary, longer incubation time, aminated surface, and smaller sized MP/NP co-cultured with PFAS increased the cytotoxicity of SNU-1 human gastric cells. |