Skip to main content
ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Bioproducts Research » Research » Publications at this Location » Publication #418616
Research Project: Zero Waste Agricultural Processing

Location: Bioproducts Research

Title: Preparative fractionation of feruloyl oligosaccharides produced by combinatorial enzyme digestion of arabinoxylan

Author
item Wong, Dominic
item Batt Throne, Sarah
item Orts, William

Submitted to: Advances in Enzyme Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/27/2024
Publication Date: 9/30/2024
Citation: Wong, D., Batt Throne, S.B., Orts, W.J. 2024. Preparative fractionation of feruloyl oligosaccharides produced by combinatorial enzyme digestion of arabinoxylan. Advances in Enzyme Research. 12(13):35-43. https://doi.org/10.4236/aer.2024.123003.
DOI: https://doi.org/10.4236/aer.2024.123003

Interpretive Summary: Non-digestible oligosaccharides have been widely promoted as prebiotics with health benefits linked to modulation of the human gut microorganisms. The combinatorial enzyme technique invented by our group has been applied to generate various functional oligosaccharides from lignocellulosic polymers. The key process involves the cooperative interaction between the main chain enzymes and accessory enzymes targeting the cleavage of side group substitutions. In the present study, species of feruloyl oligosaccharides have been obtained showing antimicrobial activities with effective inhibitory levels comparable to some common food preservatives.

Technical Abstract: Pretreated wheat insoluble arabinoxylan was converted to oligosaccharides of structural variants using combinatorial enzyme digestion. The products were separated by preparative chromatographic Amberlite XAD-2 column. Fractions containing feruloyl oligosaccharides (FOS) were isolated, pooled, freeze-dried, and demonstrated to possess antimicrobial activity. The FOS suppressed cell growth of the test organism ATCC 8739 E. coli. with a MIC value of 0.028% (w/v, 35C, 24 hr). The antimicrobial action was observed exceeding 72 hr of culture incubation. The FOS product could be a useful source of prebiotics or preservatives. The present results further confirm the science and application of the combinatorial enzyme concept.