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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #417848
Research Project: Genetic Resource and Information Management for Pulse, Temperate Forage Legume, Oilseed, Vegetable, Grasses, Sugar, Ornamental, and Other Crops

Location: Plant Germplasm Introduction and Testing Research

Title: Association study of crude seed protein and fat concentration in a USDA pea diversity panel

Author
item UHDRE, RENAN - Washington State University
item Coyne, Clarice - Clare
item Bourland, Britton
item PIASKOWSKI, JULIA - University Of Idaho
item ZHENG, PING - Washington State University
item GANJYAL, GIRISH - Washington State University
item ZHANG, ZHIWU - Washington State University
item McGee, Rebecca
item MAIN, DORRIE - Washington State University
item BANDILLO, NONOY - North Dakota State University
item MORALES, MARIO - North Dakota State University
item MA, YU - The Ohio State University
item CHEN, CHENGCI - Montana State University
item FRANCK, WILLIAM - Montana State University
item THRASH, ADAM - Mississippi State University
item Warburton, Marilyn

Submitted to: The Plant Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/2024
Publication Date: 7/31/2024
Citation: Uhdre, R., Coyne, C.J., Bourland, B.M., Piaskowski, J., Zheng, P., Ganjyal, G., Zhang, Z., McGee, R.J., Main, D., Bandillo, N., Morales, M., Ma, Y., Chen, C., Franck, W., Thrash, A., Warburton, M.L. 2024. Association study of crude seed protein and fat concentration in a USDA pea diversity panel. The Plant Genome. Article 20485. https://doi.org/10.1002/tpg2.20485.
DOI: https://doi.org/10.1002/tpg2.20485

Interpretive Summary: Peas are an important crop increasingly valued in the food industry for their protein content. This research aimed to enhance pea seed protein levels by identifying high-protein pea lines and genetic tools. Using a diverse panel of yellow-seeded peas from the USDA ARS gene bank, we conducted a detailed genetic analysis to identify genesand cellular pathways linked to higher protein and fat concentrations in pea seeds. Note that 41 high-protein lines were identified. Genes responsible for protein cellular transportation and those involved in lipid metabolism were found. This knowledge paves the way for breeding peas with high nutritional profiles, meeting agricultural and dietary needs. These findings will help breeders create improved pea cultivars for US farmers and food markets.

Technical Abstract: Pea (Pisum sativum L.) is a key rotational crop and is increasingly important in the food processing sector for its protein. This study focused on identifying diverse high seed protein concentration (SPC) lines in pea plant genetic resources. Objectives included identifying high-protein pea lines, exploring genetic architecture across environments, pinpointing genes and metabolic pathways associated with high protein, and documenting information for single nucleotide polymorphism (SNP)-based marker-assisted selection. From 2019 to 2021, a 487-accession pea diversity panel, More protein, More pea, More profit, was evaluated in a randomized complete block design. DNA was extracted for genomic analysis via genotype-by-sequencing. Phenotypic analysis included protein and fat measurements in seeds and flower color.