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ARS Home » Plains Area » Las Cruces, New Mexico » Cotton Ginning Research » Research » Publications at this Location » Publication #417564
Research Project: Improving the Production and Processing of Western and Long-Staple Cotton and Companion Crops to Enhance Quality, Value, and Sustainability

Location: Cotton Ginning Research

Title: Effect of harvesting method and location on the hydrolysis yield of dilute acid pretreated corn stover fractions

Author
item KAUR, GUNDEEP - State University Of New York- College Of Environmental Science And Forestry
item PAUL, ANINDITA - State University Of New York- College Of Environmental Science And Forestry
item ASTON, JOHN - Idaho National Laboratory
item EMERSON, RACHEL - Idaho National Laboratory
item Tumuluru, Jaya Shankar
item KUMAR, DEEPAK - State University Of New York- College Of Environmental Science And Forestry

Submitted to: ASABE Annual International Meeting
Publication Type: Other
Publication Acceptance Date: 7/29/2024
Publication Date: 7/29/2024
Citation: Kaur, G., Paul, A., Aston, J.E., Emerson, R.M., Tumuluru, J., Kumar, D. 2024. Effect of harvesting method and location on the hydrolysis yield of dilute acid pretreated corn stover fractions. 2024 ASABE Annual International Meeting, July 28-31,2024, Anaheim, California. Presentation only.

Interpretive Summary:

Technical Abstract: This study aimed to determine the effect of harvesting method and location on the cellulose conversion during enzymatic hydrolysis of dilute acid pretreated corn stover fractions. Twelve corn stover bales were collected that vary in the harvest location and method. Each bale was separated into flakes, and grab samples from flakes at each end of the bale and from the middle were selected and combined in a container. Following this, the material was manually separated into pure anatomical fractions of stalk, husk, leaf, and cob. All biomass samples were ground to particle size 2 mm. Biomass was pretreated in a high-pressure Parr reactor at 10% solid loading using dilute sulfuric acid (1% w/w) at 160 ' for 7 minutes. The pretreated slurry was separated into liquid and solid fractions via filtration, and the solids were neutralized by washing in excess water. The pretreated biomass was hydrolyzed at 50°C for 72 hours using commercial cellulase and hemicellulose enzymes at a loading of 20 mg/g biomass and 2 mg/g biomass, respectively. The sugar concentrations in the hydrolysate were determined by analyzing hydrolysate in the HPLC. The cellulose conversion was determined based on the glucose concentrations in the hydrolysate and glucans present inherently in the biomass (as per the composition of pretreated biomass). The data analysis is in progress.