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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #416863
Research Project: Characterization and Introgression of Nematode Resistance into Upland Cotton

Location: Crop Genetics Research

Title: Mapping QTLs controlling resistance of Gossypium arboreum to reniform nematode

Author
item Feng, Chunda
item Stetina, Salliana
item Erpelding, John

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/29/2024
Publication Date: 11/13/2024
Citation: Feng, C., Stetina, S.R., Erpelding, J.E. 2024. Mapping QTLs controlling resistance of Gossypium arboreum to reniform nematode. Meeting Abstract. https://scisoc.confex.com/scisoc/2024am/meetingapp.cgi/Paper/160557.

Interpretive Summary:

Technical Abstract: Reniform nematode (Rotylenchulus reniformis) is an important parasite of cotton (Gossypium hirsutum), which causes severe yield loss to U.S. cotton production. Nematicides and crop rotation are common practices to manage this disease, but not very effective. Growing resistant cultivars could provide season-long protection. However, resistance was not found in widely grown Upland cotton, but found in Sea Island cotton and several diploid Gossypium species such as G. arboreum. The G. arboreum resistant accession A2-87 (PI 417895) and susceptible accession A2-101 (PI 529729) were used to develop a F2 population. The individual F2 plants, the two parental lines, and a susceptible control Deltapine 16, were inoculated with reniform nematodes following an established protocol. The number of female nematodes and the fresh root weight of each plant were recorded to calculate two female indices, pctF (percentage of the number of female nematodes of a plant compared to that of the susceptible control) and pctFPG (percentage of the number of female nematodes per gram fresh root of a plant compared to that of the susceptible control). The two parents and individual F2 plants were sequenced using the genotyping-by-sequencing approach. Variants were called with software bcftools. A total of 4449 SNPs that were different between the two parents and segregating at 1:2:1 ratio was employed for detecting quantitative trait loci (QTLs) controlling nematode resistance with the composite interval mapping method in software WinQTLcart 2.5. Six QTLs were detected on chromosome 10 for pctF (LOD=3.0), with five of them located within a 15 cM interval. For pctFPG, one QTL each was found on chromosomes 3 and 7, and three QTLs on chromosome 10 within a 12 cM interval. The QTL-containing intervals for both pctF and pctFPG were overlapped on chromosome 10. The QTL regions will be further investigated for disease resistance genes.