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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #416747
Research Project: Improving Salmonid Health through Breeding, Vaccination and Microbiome Modulation

Location: Cool and Cold Water Aquaculture Research

Title: Pseudomonas aphyarum sp. nov., Pseudomonas fontis sp. nov., Pseudomonas idahonensis sp. nov. and Pseudomonas rubra sp. nov., isolated from in, and around, a rainbow trout farm

Author
item GRAF, JOERG - University Of Connecticut
item TESTERMAN, TODD - University Of Connecticut
item VARGA, JACKIE - University Of Connecticut
item DONOHUE, HAILEY - University Of Connecticut
item VIEIRA DA SILVA, CAROLINE - University Of Connecticut
item SCHIFFER, MOLLY - University Of Hawaii

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2023
Publication Date: 12/18/2023
Citation: Graf, J., Testerman, T., Varga, J., Donohue, H., Vieira Da Silva, C., Schiffer, M. 2023. Pseudomonas aphyarum sp. nov., Pseudomonas fontis sp. nov., Pseudomonas idahonensis sp. nov. and Pseudomonas rubra sp. nov., isolated from in, and around, a rainbow trout farm. International Journal of Systematic and Evolutionary Microbiology. 73(12):006201. https://doi.org/10.1099/ijsem.0.006201.
DOI: https://doi.org/10.1099/ijsem.0.006201

Interpretive Summary: Pseudomonas species are wide-spread in the aquatic environment. Members of this genus can be pathogens of animals and plants but are also known to produce antibiotics. In this study, we cultured bacteria from biofilms at a large commercial trout farm or its water source in Idaho. Here we report and name four new Pseuodomonas species. Their genomes were sequenced which allows us to search for gene clusters important in antibiotic production. This study will lay the foundation of a more detailed analysis of these strains, which is important as members of these four species inhibit the growth of several fish pathogens.

Technical Abstract: For this study, we cultured ~1,000 isolates from a fish farm and its water source in Idaho. ~600 isolates were identified by PacBio sequencing the 16S rRNA gene and tested for their ability to inhibit the growth of other bacteria. The 16S rRNA gene suggested that we had 10 strains belonging to undescribed species of Pseudomonas. Whole genome analysis confirmed this finding and we described Pseudomonas aphyarum sp. nov., Pseudomonas fontis sp. nov., Pseudomonas idahonensis sp. nov. and Pseudomonas rubra sp. nov.. Further phenotypic characterization using API 20NE, API ZYM and Biolog GENIII assays and chemotaxonomic analysis of cellular fatty acids were carried out. The type strains were deposited to ATCC and DSMZ.