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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Toxicology & Mycotoxin Research » Research » Publications at this Location » Publication #416119
Research Project: Strategies to Reduce Mycotoxin Contamination in Animal Feed and its Effect in Poultry Production Systems

Location: Toxicology & Mycotoxin Research

Title: Characterization of certain bioactive compounds extracted from bacteria versus Biomphalaria alexandrina

Author
item MEKAWEY, AMAL - Al-Azhar University
item SALAH, AHMED - Al-Azhar University
item ALQHTANI, ABDULMOHSEN - King Saud University
item YOSRI, MOHAMMED - Al-Azhar University
item Pokoo-Aikins, Anthony

Submitted to: Polish Journal of Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2024
Publication Date: 8/21/2025
Citation: Mekawey, A.A., Salah, A.M., Alqhtani, A.H., Yosri, M., Pokoo-Aikins, A. 2025. Characterization of certain bioactive compounds extracted from bacteria versus Biomphalaria alexandrina. Polish Journal of Microbiology. https://doi.org/10.15244/pjoes/204566.
DOI: https://doi.org/10.15244/pjoes/204566

Interpretive Summary: The parasite illness of schistosomiasis causes harm to organs in human. An effective way to control this disease is snail management. The goal of this study was to find bacterial types that were most poisonous to Biomphalaria alexandrina (BA). BA is the main host for Schistosoma manosni. 24 bacterial type cultures were obtained and exposed to snails. The snails were taken out of the bacterial concentration and evaluated at the end of the exposure time. The snails were rinsed with water and placed in different containers for 24-hour to recover. The number of snails that died were noted to identify the most poisonous bacterial type. Testing showed that different bacterial extracts had different rates of snail death when applied to BA. The most lethal substance against the snails were from S. aureus and E. coli. They contained active compounds with maximum toxicity against BA. The isolates are being used for further investigation.

Technical Abstract: The parasite illness schistosomiasis causes significant harm to the organs in human upon infection. The most effective strategy for schistosomiasis management is snail management. The current study's goal was to determine the bacterial species that are most poisonous to Biomphalaria alexandrina (B. alexandrina) as potential host for Schistosoma manosni (S. manosni). 24 bacterial filtrates were applied to the snails for 24 hours to examine their impact on percentages of snails' mortality at a level of 100 ppm. Molluscicidial impact of the most efficient compounds derived from bacterial filtrates expressed as (LC10, LC25, LC50, and LC90). The effects of promising compounds were evaluated versus Daphnia pulex (D. pulex) to investigate their toxic impact. Biochemical parameters were evaluated to test the impact of the most promising purified compounds. Based on H1-NMR, FTIR, and mass data, the proposed chemical structures of the isolated compounds were Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) filtrates have the most effective impact towards B. alexandrina. Sub-lethal doses (LC10 and LC25) of purified compounds were reported to have dramatic impact on liver enzymes and minimal impact on other tested parameters. A notable variation in the protein pattern of the treated snails using efficient bacterial molecules versus control. The derived compounds have been reported to have minimal toxicity on D. pulex. The proposed name of identified compound from S. aureus was (5-sec-Butyl-4,5,6,7-tetrahydro-1H-indol-3-yl)- acetaldehyde, while, The proposed name of identified compound from E. coli was 2-Isobutyl-6-(4-methyl-pentyl)-phenylamine to be produced for future potential uses.