Complete hybrid genome assembly of mannheimia haemolytica serotype A2 strain D95 isolated from ovine lung

Authors: Goldkamp, Anna; MENGHWAR, HARISH - Oak Ridge Institute For Science And Education (ORISE); Dassanayake, Rohana; Tatum, Fred; Briggs, Robert; Casas, Eduardo

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/16/2024
Publication Date: 10/14/2024
Citation: Goldkamp, A.K., Menghwar, H., Dassanayake, R.P., Tatum, F.M., Briggs, R.E., Casas, E. 2024. Complete hybrid genome assembly of mannheimia haemolytica serotype A2 strain D95 isolated from ovine lung. Microbiology Resource Announcements. Article e0055224. https://doi.org/10.1128/mra.00552-24.
DOI: https://doi.org/10.1128/mra.00552-24

Interpretive Summary: Mannheimia haemolytica is a costly bacteria that is mainly associated with respiratory disease in cattle, sheep, and other ruminants. In a stressed or immunocompromised animal, M. haemolytica can spread and establish infection in the lower respiratory tract. Serotype 2 is predominantly isolated from pneumonic sheep lung. Whole genome sequencing was conducted in a serotype 2 strain isolated from sheep lung. Comparison of ovine and cattle serotype 2 genomes from pneumonic lung will allow scientists to improve disease intervention strategies.

Technical Abstract: Mannheimia haemolytica is the most important bacterial pathogen associated with broncho- and fibrinous-pneumonia in ruminants. Here, we report the complete genome sequence of an isolate of serotype A2 M. haemolytica (D95) recovered from pneumonic ovine lung. The D95 genome has a size of 2.7 Mb and contains 2,723 genes.