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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #415387
Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Discovering bacterial leaf streak disease resistance loci in barley in the interspecific Cytonuclear Multi-Parent Population (CMPP)

Author
item TILSTRA, JOSEPH - South Dakota State University
item AHMAD, MUHAMMAD - South Dakota State University
item PAWAR, TAPISH - South Dakota State University
item BEERY, AVITAL - Volcani Center (ARO)
item BODENHEIMER, SCHEWACH - Volcani Center (ARO)
item Yang, Shengming
item SOLANKI, SHYAM - South Dakota State University
item FRIDMAN, EYAL - Volcani Center (ARO)
item AMEEN, GAZALA - South Dakota State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2024
Publication Date: 6/10/2024
Citation: Tilstra, J., Ahmad, M., Pawar, T., Beery, A., Bodenheimer, S., Yang, S., Solanki, S., Fridman, E., Ameen, G. 2024. Discovering bacterial leaf streak disease resistance loci in barley in the interspecific Cytonuclear Multi-Parent Population (CMPP). Meeting Abstract. 2024 Annual Meeting of the North Central Division of the American Phytopathological Society.

Interpretive Summary:

Technical Abstract: A noticeable impact of climate change is the outbreak of emerging diseases. One such disease that was not new to the US but has become predominant in the Northern Great Plains is Bacterial leaf streak or BLS. BLS is a bacterial disease of barley, wheat, and oats caused by Xanthomonas translucens. The initial disease symptoms are water-soaking and progressing into large necrotic streaks on leaves. BLS can also infect barley spikes causing black chaff symptoms reducing yield and rendering inferior-quality barley grains that cannot be used for malting. Due to the absence of resistant varieties and limited chemical or biological control, it is essential to find and deploy host resistance in the current, high-yielding varieties. In this study, we screened 692 doubled haploid lines belonging to the interspecific Cytonuclear Multi-Parent Population (CMPP) to discover marker-trait association (MTAs) for BLS resistance. We used spray inoculations and recorded the disease phenotypic data using our 1 to 9 BLS disease rating scale. The CMPP population was genotyped using the 50K illumina Infinium chip. After quality control, we used 19,988 SNP markers for a genome-wide association study and used MLM, GLM, Blink, and farmCPU models to find MTAs linked to BLS resistance. Our results found significant MTAs on chromosomes 1H, 5H, and 7H. Ongoing field testing will validate our results to confirm the BLS genetic resistance in these identified genomic regions.