Combination and individual vaccines for bovine viral diarrhea virus and infectious bovine rhinotracheitis effects on reproductive cyclicity and immune response

Authors: BLASKE, SARAH - Texas A&M Agrilife; KETCHUM, JACLYN - Texas A&M Agrilife; QUAIL, LACY - Texas A&M Agrilife; MATTOS, ANA CLARA - University Of São Paulo; Snider, Alexandria - Alex; CARSON, CHLOEY - Texas A&M Agrilife; LONG, CHARLES - Texas A&M Agrilife; PERRY, GEORGE - Texas A&M Agrilife

Submitted to: American Society of Animal Science Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/25/2024
Publication Date: 9/13/2024
Citation: Blaske, S., Ketchum, J.N., Quail, L.K., Mattos, A.C., Snider, A.P., Carson, C.P., Long, C.R., Perry, G.A. 2024. Combination and individual vaccines for bovine viral diarrhea virus and infectious bovine rhinotracheitis effects on reproductive cyclicity and immune response (abstract). American Society of Animal Science Annual Meeting. 102(3):269-270. https://doi.org/10.1093/jas/skae234.310.
DOI: https://doi.org/10.1093/jas/skae234.310

Interpretive Summary:

Technical Abstract: Vaccines are known to impact both the immune and reproductive systems. The objective of this research was to identify immunological and cyclic differences following vaccination for Bovine Viral Diarrhea Virus (BVDV) and/or Infectious Bovine Rhinotracheitis (IBR). Brahman cows (n=15) were administered PGF2a to regress corpus lutea on d-3. Animals (d0) were immunized (2mL I.M.) with one of three treatments: 1) Modified Live Vaccine (MLV) for BVDV and IBR (BVD+IBR; n = 5), 2) MLV for BVDV only (BVD; n = 5), or 3) sterile saline (CON; n = 5). Blood samples (30 mL/cow) were collected (d-3, 0, 2, 4, 6, 8, 10, and 14) and peripheral blood mononuclear cells (PBMC) were isolated. PBMCs were incubated with propidium iodide and antibodies for specific surface cell markers (CD4, CD8, CD25, CD14, CD86, and CD335). An Amnis FlowSight flow cytometer determined cell type percentage. BVDV and IBR antibody concentrations were analyzed in serum samples (d-3, 0, and 14). Plasma samples (d0, 2, 4, 6, 8, 10, and 14) were evaluated for IFN-', IL-1a, IL-1ß, IL-4, IL-6, IL-8, IL-10, IL-17A, MIP-1a, MIP-1ß, IL-36RA, IP-10, MCP-1, TNFa, and VEGFA concentrations by a MagPix multiplex machine using a MILLIPLEX Bovine Cytokine/Chemokine Magnetic Bead Panel. Progesterone (d0, 2, 4, 6, 8, 10, and 14) and estradiol (d0) concentrations were determined by RIA. Differences in antibody titers, PBMC populations, and cytokine concentrations were analyzed as repeated measures using PROC MIXED (SASv9.4). Forty percent of BVD+IBR animals experienced abnormal estrus cycles, but no BVD or CON animals did. There was a treatment effect on BVD titer concentration with BVD animals having greater titer concentrations than CON (P=0.02) but similar concentrations to BVD+IBR (P=0.18). There was a treatment by time interaction (P<0.0001) on IBR titers with BVD+IBR animals having greater titer concentrations on d14 compared to BVD and CON. Treatment had no effect on leukocyte populations (P>/=0.1409), but all populations differed over time (P