Registration of four durum wheat lines carrying Sr13 alleles for resistance to stem rust

Authors: Klindworth, Daryl; Rouse, Matthew; OLIVERA, PABLO - University Of Minnesota; Jin, Yue; Chu, Chenggen; Friesen, Timothy; ZHONG, SHAOBIN - North Dakota State University; Faris, Justin; Fiedler, Jason; Peters Haugrud, Amanda; Gu, Yong; ELIAS, ELIAS - North Dakota State University; LIU, SHUYU - Texas A&M Agrilife; Cai, Xiwen; Xu, Steven

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/26/2024
Publication Date: 12/3/2024
Citation: Klindworth, D.L., Rouse, M.N., Olivera, P., Jin, Y., Chu, C.N., Friesen, T.L., Zhong, S., Faris, J.D., Fiedler, J.D., Peters Haugrud, A.R., Gu, Y.Q., Elias, E.M., Liu, S., Cai, X., Xu, S.S. 2024. Registration of four durum wheat lines carrying Sr13 alleles for resistance to stem rust. Journal of Plant Registrations. 19(1). Article e20399. https://doi.org/10.1002/plr2.20399.
DOI: https://doi.org/10.1002/plr2.20399

Interpretive Summary: Stem rust resistance gene Sr13 is one of the most important genes in durum wheat for resistance to stem rust, which is one of the most destructive diseases in durum and bread wheat. Four Sr13 variants, namely alleles Sr13a, Sr13b, Sr13c, and Sr13d, were previously identified from durum wheat and its related wheat subspecies. In this study, four durum wheat lines Rusty-KLB, Rusty-14803, Rusty-ST464C1, and CATA1 carrying stem rust resistance alleles Sr13a, Sr13b, Sr13c, and Sr13d, respectively, were developed and released as new durum wheat germplasm for stem rust resistance. These lines are useful for wheat geneticists and pathologists in studying the host-pathogen interactions of wheat stem rust and for wheat breeders for breeding durum and bread wheat with stem rust resistance.

Technical Abstract: Four durum wheat (Triticum turgidum ssp. durum) lines Rusty-KLB, Rusty-14803, Rusty-ST464C1, and CATA1, which carry stem rust resistance gene Sr13 alleles Sr13a, Sr13b, Sr13c, and Sr13d, respectively, are released by USDA-ARS. These alleles originated from cultivated emmer wheat (T. turgidum ssp. dicoccum) landrace Khapli (CItr 4013), T. turgidum ssp. polonicum accession CItr 14803, durum landrace ST464 (PI 191365), and durum line Camadi Abdu tipo #103 (PI 192168), respectively. Rusty-KLB, Rusty-14803, and Rusty-ST464C1 are near-isogenic lines (NILs) with the pedigrees Rusty*7/KL-B, Rusty*4/3/Rusty/CItr 14803//2*Rusty, and Rusty*7/ST464-C1, respectively. KL-B, ST464-C1, and CATA1 are monogenic lines with the pedigrees Marruecos 9623//Khapli/Marruecos 9623, Marruecos 9623*2/ST464, and Marruecos 9623*2/Camadi Abdu tipo #103, respectively. Sr13 can be detected by perfect markers including Kompetitive allele specific PCR (KASP) marker KASPSr13 and semi-thermal asymmetric reverse PCR (STARP) markers rwgsnp37. Specific alleles can be identified via STARP markers rwgsnp38, rwgsnp39, and rwgsnp40. The Sr13 alleles provide a moderate level of resistance, typically an infection type (IT) 2, to a broad spectrum of stem rust races. Sr13c was effective against all 15 stem rust races tested while Sr13a was ineffective only against race TCMJC. Sr13b was ineffective against JRCQC, QCCJC, QFCSC and TTRTF. Sr13d is notable in being the only Sr13 allele ineffective to TTKSK (Ug99) and TKTTF. These lines are useful for studying the host-stem rust pathogen interactions, identifying new Sr genes, and breeding durum and bread wheat with stem rust resistance.