Skip to main content
ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Publications at this Location » Publication #412887
Research Project: Crop Health and Genetic Improvement of Sub-Tropical and Tropical Crops in the Pacific

Location: Tropical Plant Genetic Resources and Disease Research

Title: High-Quality complete genome sequence of xanthomonas phaseoli pv. dieffenbachiae outbreak strain D182: The causative agent of anthurium bacterial blight in Hawaii

Author
item DOBHAL, SHEFALI - University Of Hawaii
item CHUANG, SHU-CHENG - University Of Hawaii
item ARIZALA, DARIO - University Of Hawaii
item Keith, Lisa
item ALVAREZ, ANNE - University Of Hawaii
item ARIF, MOHAMMAD - University Of Hawaii

Submitted to: PhytoFrontiers
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/13/2024
Publication Date: 7/10/2024
Citation: Dobhal, S., Chuang, S., Arizala, D., Keith, L.M., Alvarez, A.M., Arif, M. 2024. High-Quality complete genome sequence of xanthomonas phaseoli pv. dieffenbachiae outbreak strain D182: The causative agent of anthurium bacterial blight in Hawaii. PhytoFrontiers. 4:812-816. https://doi.org/10.1094/PHYTOFR-02-24-0006-A.
DOI: https://doi.org/10.1094/PHYTOFR-02-24-0006-A

Interpretive Summary: Bacterial blight of anthurium caused by Xanthomonas phaseoli pv. dieffenbachiae (Xpd) (formerly known as Xanthomonas axonopodis pv. dieffenbachiae) is regarded as one of the most devastating diseases, causing significant crop losses in commercial foliage plant production and cut flowers. Due to the economic importance of Anthurium cut flowers in the European Union, Xpd is listed as an EPPO A2 quarantine pathogen. The epidemic in Hawaii reached its peak between 1985 and 1989. In this study, the complete genome of Xpd strain D182, isolated in 1986 from an anthurium plant infected during the outbreak, was sequenced.

Technical Abstract: Xanthomonas phaseoli pv. dieffenbachiae (Xpd), the causal agent of anthurium blight, is classified as an A2 quarantine organism on the EPPO list due to its devastating impact on the anthurium industry. In this study, we sequenced strain D182, representative of the Hawaiian anthurium blight outbreak (1981-1986), using PacBio RS II SMRT technology. High-quality de novo assembly of 5,217,888 bp (65% GC) was generated with a mean coverage of 351 using HGAP v4. Strain D182 harbors one plasmid (73.5 kb, GC – 60.8%). ANI and dDDH values, which ranged from 99.68-99.86% and 96.8-98.9% respectively, showed close phylogenetic relationships with Xpd strain LMG 695. The genome information will be useful in providing insights into the genomic biology, virulence mechanisms, and evolutionary relationships of Xpd and other strains associated with anthurium blight outbreaks worldwide.