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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Mycology and Nematology Genetic Diversity and Biology Laboratory » Research » Publications at this Location » Publication #412373

Research Project: Plant-associated Nematode Management and Systematics and USDA Nematode Collection Curation

Location: Mycology and Nematology Genetic Diversity and Biology Laboratory

Title: Genome announcement: draft genome assembly of Heterodera humuli generated using long-read sequencing

Author
item NUNEZ-RODRIGUEZ, LESTER - Oregon State University
item Wram, Catherine
item Hesse, Cedar
item Zasada, Inga

Submitted to: Journal of Nematology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/5/2024
Publication Date: 8/27/2024
Citation: Nunez-Rodriguez, L.A., Wram, C.L., Hesse, C.N., Zasada, I.A. 2024. Genome announcement: draft genome assembly of Heterodera humuli generated using long-read sequencing. Journal of Nematology. 56(1). Article 20240029. https://doi.org/10.2478/jofnem-2024-0029.
DOI: https://doi.org/10.2478/jofnem-2024-0029

Interpretive Summary: Plant-parasitic nematodes are microscopic round worms that pose a significant problem to crop production globally; resulting in more than $110 billion in crop loss annually. The main nematode threatening hop production is the Hop Cyst Nematode, Heterodera humuli. In order to develop management strategies for this nematode it is necessary to gain a better understanding of its basic biology; therefore, the goal of this research was to sequence the genome of this nematode. This genome will be a helpful resource to other scientists studying specific genes and proteins found in the Hop Cyst Nematode and other cyst nematodes that infect a variety of crops like soybean, wheat, carrot, and sugar beets.

Technical Abstract: The hop cyst nematode, Heterodera humuli is the most common plant-parasitic nematode associated with hop worldwide. This study reports the draft genome of H. humuli generated on the PacBio Sequel IIe System with the ultra-low DNA input HiFi sequencing method, and the corresponding genome annotation. This genome resource will help further studies on H. humuli and other cyst nematodes.