Genome-wide mapping of quantitative trait loci conferring resistance to stripe rust in spring wheat line PI 660072

Authors: ZHOU, XINLI - Southwest University Of Science And Technology; WANG, YUQI - Southwest University Of Science And Technology; LUO, YUQI - Southwest University Of Science And Technology; SHUAI, JIE - Southwest University Of Science And Technology; JIA, GUOYUN - Southwest University Of Science And Technology; CHEN, HONGYANG - Southwest University Of Science And Technology; ZHANG, LIANGQI - Southwest University Of Science And Technology; CHEN, HAO - Southwest University Of Science And Technology; LI, XIN - Southwest University Of Science And Technology; HUANG, KEBING - Southwest University Of Science And Technology; YANG, SUIZHUANG - Southwest University Of Science And Technology; WANG, MEINAN - Washington State University; REN, YONG - Southwest University Of Science And Technology; LI, GANG - Nanjing Agricultural University; Chen, Xianming

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/9/2024
Publication Date: 10/23/2024
Citation: Zhou, X., Wang, Y., Luo, Y., Shuai, J., Jia, G., Chen, H., Zhang, L., Chen, H., Li, X., Huang, K., Yang, S., Wang, M., Ren, Y., Li, G., Chen, X. 2024. Genome-wide mapping of quantitative trait loci conferring resistance to stripe rust in spring wheat line PI 660072. Theoretical and Applied Genetics. 2024(137). Article 255. https://doi.org/10.1007/s00122-024-04760-4.
DOI: https://doi.org/10.1007/s00122-024-04760-4

Interpretive Summary: Stripe rust is one of the most devastating diseases of wheat worldwide. Identifying resistance genes is crucial for developing resistant cultivars to control the disease. Spring wheat PI 660072 has been identified to possess both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust. To elucidate the genetic basis of the resistance in PI 660072, a mapping population consisting of 211 recombinant inbred lines (RILs) was developed from a cross of PI 660072 with susceptible spring wheat Avocet S. The mapping population was phenotyped for stripe rust responses across five field environments from 2020 to 2022 and genotyped using the 15K SNP (single nucleotide polymorphism) array to map stripe rust resistance loci. The mapping population was also tested at the seedling stage with predominant Chinese races CYR31, CYR32, CYR34 and Pst-YX1-3-1 of the pathogen in the greenhouse. Stripe rust resistance genes were identified using the quantitative trait locus (QTL) mapping approach. Two QTL were mapped, one on the long arm of chromosome 2B for ASR and one on the long arm of chromosome 4B for APR. To facilitate marker-assisted selection breeding, Kompetitive allele specific PCR (KASP) markers were developed. These markers could be used to introgress the effective resistance QTL into new wheat cultivars.

Technical Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat worldwide. Identifying resistance genes is crucial for developing resistant cultivars to control the disease. Spring wheat PI 660072 (Triticum aestivum) has been identified to possess both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust. To elucidate the genetic basis of the resistance in PI 660072, a mapping population consisting of 211 F5 - F7 recombinant inbred lines (RILs) was developed from a cross of PI 660072 with susceptible spring wheat Avocet S. The mapping population was phenotyped for stripe rust responses across five field environments from 2020 to 2022 and genotyped using the 15K SNP (single nucleotide polymorphism) array to map stripe rust resistance loci. The mapping population was also tested at the seedling stage with predominant Chinese Pst races CYR31, CYR32, CYR34 and Pst-YX1-3-1 in the greenhouse. Stripe rust resistance genes were identified using the quantitative trait locus (QTL) mapping approach. Two QTL were identified with QYrPI660072.swust-2BL mapped on the long arm of chromosome 2B for ASR and QYrPI660072.swust-4BL on the long arm of chromosome 4B for APR. To facilitate marker-assisted selection breeding, Kompetitive allele specific PCR (KASP) markers, KASP-1269 for QYrPI660072.swust-2BL and KASP-3209 for QYrPI660072.swust-4BL, were developed. These markers could be used to introgress the effective resistance QTL into new wheat cultivars.