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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #411244
Research Project: Genetic Resource and Information Management for Pulse, Temperate Forage Legume, Oilseed, Vegetable, Grasses, Sugar, Ornamental, and Other Crops

Location: Plant Germplasm Introduction and Testing Research

Title: Insights on the regulatory landscape of developing pod in field pea

Author
item JOHNSON, JOSEPHINE - North Dakota State University
item KIM, JEONGHWA - North Dakota State University
item ISSAKA, OUSSEINI - Washington State University
item SHREEJANA, K - North Dakota State University
item MORALES, MARIO - North Dakota State University
item SALUDARES, RICA - North Dakota State University
item ACHARYA, SHAILESH - North Dakota State University
item NAVASCA, HARRY - North Dakota State University
item PICHE, LISA - North Dakota State University
item WORRAL, HANNAH - North Dakota State University
item SARI, HATICE - Washington State University
item MA, YU - Washington State University
item MAIN, DORRIE - Washington State University
item MCPHEE, KEVIN - Montana State University
item Coyne, Clarice
item McGee, Rebecca
item BANDILLO, NONOY - North Dakota State University

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/1/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary: Determining the genes that encode traits of interest allow scientists to create new, improved crop varieties for those traits. One way of identifying genes that encode key traits is transcriptome-wide association study (TWAS), a relatively new approach that compares how often a gene is copied into a protein for plants with a good level of the trait of interest vs. plants with a low level of the trait. This study found genes that were highly copied in pea plants that developed many seed pods rapidly, with high protein levels, compared to plants with fewer and slower growing pods and lower protein levels. Transcriptome-wide association study detected genes for both seed yield and seed protein. Future analysis will try to use the genes to create higher yielding, more nutritious pea varieties.

Technical Abstract: Transcriptome-wide association study (TWAS) is a relatively new approach using gene expression differences to identify genes encoding key traits for crop improvement. Our objectives were the development of gene expression resources for the pea pod development trait, quantify the regulatory variants in gene expression profiles, and map the trait-associated genes using transcriptome-wide association study. Three hundred pea accessions were grown in 2022 and optimized pod-harvest sampling was developed and completed for 3 environments: North Dakota, Montana and Washington. Isolated mRNA was sequenced and quantified, and expression data analyzed for two locations (environments). TWAS detected common and unique sets of genes for both seed yield and seed protein between North Dakota and Washington. Future analysis will be conducted for accounting GXE using more environments with the TWAS framework.