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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #410408

Research Project: Genetic Improvement of Sorghum Traits that Advance Agricultural Productivity and Climate Resilience

Location: Plant Stress and Germplasm Development Research

Title: A large Sequenced Mutant library provides gene leads for breeding and genome editing

item Xin, Zhanguo
item JIAO, YINPING - Texas Tech University
item Burow, Gloria
item Hayes, Chad
item Chen, Junping
item Pugh, Nicholas - Ace
item Ware, Doreen

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/13/2023
Publication Date: 1/14/2024
Citation: Xin, Z., Jiao, Y., Burow, G.B., Hayes, C.M., Chen, J., Pugh, N.A., Ware, D. 2024. A large Sequenced Mutant library provides gene leads for breeding and genome editing. Plant and Animal Genome Conference.

Interpretive Summary:

Technical Abstract: Induced mutagenesis is a powerful approach to generate variations for elucidation of gene function and create new traits for breeding. We have developed a pedigreed mutant library through chemical mutagenesis with ethylmethane sulfonate (EMS) treated seeds in sorghum and sequenced 1256 randomly selected lines. The mutant library displayed many traits of significant agronomic value. This endeavor resulted in detection and cataloguing of over 10 million canonical EMS-induced mutations (or variants), covering over 98% of the genes in the genome of inbred line BTx623. Additionally, 610,320 mutations were identified in the promoter and enhancer regions of 18,000 and 11,790 genes, respectively. In addition, we have established BSAseq bioinformatic workflow for efficient identification of causal gene mutations underlying important traits. Mutations in genes of interest can be searched online through SorghumBase ( or SorbMuDB ( ). Our sequence-indexed sorghum mutant population provides an excellent platform to discover gene functions in the grass family, supporting plant biology research and crop breeding.