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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Grain Quality and Structure Research » Research » Publications at this Location » Publication #410347
Research Project: Measurement and Improvement of Hard Winter Wheat End-Use Quality Traits

Location: Grain Quality and Structure Research

Title: In-vitro antioxidant properties of wheat bran extracts and their inhibitory effects on collagenase, elastase, and hyaluronidase

Author
item LI, CHENG - Kansas State University
item Tilley, Michael
item Chen, Yuanhong
item SUN, XIUZHI SUSAN - Kansas State University
item WANG, WEIQUN - Kansas State University
item LI, YONGHUI - Kansas State University

Submitted to: ACS Food Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/10/2024
Publication Date: 8/16/2024
Citation: Li, C., Tilley, M., Chen, Y., Sun, X., Wang, W., Li, Y. 2024. In-vitro antioxidant properties of wheat bran extracts and their inhibitory effects on collagenase, elastase, and hyaluronidase. ACS Food Science and Technology. 4(8):1960-1966. https://doi.org/10.1021/acsfoodscitech.4c00310.
DOI: https://doi.org/10.1021/acsfoodscitech.4c00310

Interpretive Summary: Natural antioxidants, specifically phenolic compounds and bioactive peptides obtained through dietary sources, have gained interest for their potential to modulate cellular oxidative stress. In this study, arabinoxylan and proteins from two types of wheat bran were extracted and subjected to enzymatic hydrolysis to obtain xylo-oligosaccharides (XOS) and protein hydrolysates, respectively. Then, antioxidant capacities of XOS and protein hydrolysates were characterized, and their inhibitory effects were evaluated against collagenase, elastase, and hyaluronidase. The results demonstrated that the XOS and protein hydrolysates, obtained after proper extraction and enzymatic hydrolysis, exhibited promising antioxidant capacity and pronounced inhibition of the aforementioned enzymes. The total phenolic contents of XOS extracted from hard and soft wheat bran were determined to be 39.63 and 32.06 mg GAE/g, respectively. The XOS derived from hard wheat bran showed the highest hyaluronidase inhibition activity, reaching 49.9% at 0.5 mg/mL concentration among samples. The wheat bran extracts possess great potential to be used in the production of functional ingredients aimed at antioxidant and cosmetic application purposes.

Technical Abstract: The accumulation of reactive oxygen and nitrogen species (RONS) during cellular activity leads to oxidative stress, resulting in damage to cellular macromolecules, which is closely linked to aging and diseases. Natural antioxidants, specifically phenolic acids and bioactive peptides obtained through dietary sources, have gained interest for their potential to modulate cellular oxidative status. In this study, Arabinoxylan (AX) and proteins from two types of wheat bran were extracted and subjected to enzymatic hydrolysis to obtain xylo-oligosaccharides (XOS) and protein hydrolysate, respectively. Then antioxidant capacities of XOS and protein hydrolysates were characterized and their inhibitory effects on aging were evaluated through in vitro enzymatic activities using collagenase, elastase, and hyaluronidase. The results demonstrated that the XOS and protein hydrolysate, obtained after a proper extraction and enzymatic hydrolysis, exhibited significantly higher antioxidant capacity and pronounced inhibition of the aforementioned aging-related enzymes compared to previous studies. The xylo-oligosaccharides derived from hard wheat bran showed the highest hyaluronidase inhibition activity, reaching 49.9% at 0.5 mg/mL concentration, resulting in notable anti-skin aging efficacy. This study contributes to the evaluation and utilization of XOS and protein hydrolysis from wheat bran for the production of functional ingredients aimed at antioxidant and anti-aging purposes.