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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Publications at this Location » Publication #409277
Research Project: Exploiting Genetic Diversity through Genomics, Plant Physiology, and Plant Breeding to Increase Competitiveness of U.S. Soybeans in Global Markets

Location: Soybean and Nitrogen Fixation Research

Title: Comparative wheat phosphoproteome profiling pinpoints high temperature-associated breeding markers

Author
item FONSECA DE LIMA, CÁSSIO FLÁVIO - Ghent University
item ZHU, TINGTING - Ghent University
item VAN DEN BROECK, LISA - North Carolina State University
item VAN DE COTTE, BRIGITTE - Ghent University
item Locke, Anna
item SOZZANI, ROSANGELA - North Carolina State University
item DE SMET, IVE - Ghent University

Submitted to: Plant Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/26/2025
Publication Date: N/A
Citation: N/A

Interpretive Summary: Plant temperature responses are complex, and new research strategies are needed to understand these responses and improve crop heat tolerance. Here, molecular regulators controlling wheat heat responses were identified by comparing multiple wheat genotypes across multiple temperatures. A set of phosphorylated proteins phosphorylations were identified that indicate greater heat tolerance in certain wheat genotypes. One of these proteins is TOI4/5, which has also been identified as central to temperature responses in Arabidopsis and soybean. In the future, this strategy can be used to improve heat tolerance in other crops.

Technical Abstract: Rising temperatures due to climate change are adversely impacting natural and crop ecosystems, necessitating the development of heat-tolerant crops. We established a framework to pinpoint potential phosphosites in wheat varieties with varying temperature sensitivities. Our findings demonstrate that a selected set of biomarkers' phosphorylation state forms a distinctive phosphoprotein signature for heat stress tolerance. This aids in identifying targets for breeding or genome editing to enhance heat tolerance.