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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #409219
Research Project: Genetic Resource and Information Management for Pulse, Temperate Forage Legume, Oilseed, Vegetable, Grasses, Sugar, Ornamental, and Other Crops

Location: Plant Germplasm Introduction and Testing Research

Title: Genetic architecture of chickpea seed protein concentration

Author
item SARI, HATICE - Washington State University
item UHDRE, RENAN - Washington State University
item Coyne, Clarice
item Bourland, Britton
item ZHANG, ZHIWU - Washington State University
item RUSSO, MEGAN - Washington State University
item Kiszonas, Alecia
item Wallace, Lyle
item Warburton, Marilyn

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/30/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary: Chickpea is one of the most important food legumes worldwide, renowned for its high nutritional value. In this study, we investigated the genetic basis of protein concentration in 88 diverse kabuli chickpea lines from the USDA ARS collections. Protein levels were measured from seeds of each line and analyzed with genetic markers that covered the all chromosomes of chickpea. The analysis revealed 2 regions of the chromosomes associated with higher seed protein. The genes in these regions were then studied to see how they might be contributing to higher protein levels. Studying the significant regions on chromosome 4 and 6 contribute to our understanding of the genetic basis underlying the variation in nutritional components. This study provides new genetic tools to help speed the breeding efforts focused on enhancing the nutritional value of chickpea.

Technical Abstract: Chickpea (Cicer arietinum L.) is one of the most important food legumes worldwide, renowned for its high nutritional value. In this study, we investigated the genetic basis of protein concentration in the USDA kabuli chickpea mini-core collection consisting of 88 diverse accessions. Phenotyping was performed using a randomized complete block design (RCBD) with four replicates for two years for the protein concentration, and protein analyses were conducted using Near-Infrared Spectroscopy (NIR). The population was genotyped using 165,654 single nucleotide polymorphism (SNPs) derived from genotyping-by-sequencing (GBS) for all 88 individuals. Genome wide association study was conducted using the BLINK model method implemented in the GAPIT program. Two SNPs were found to be significantly associated with seed protein concentration. Significant SNPs on chromosome 4 and 6 explained 27.6% and 27% of the phenotypic variance, respectively. These findings contribute to our understanding of the genetic basis underlying the variation in nutritional components. This study provides valuable insights into the genetic factors influencing protein concentration in chickpea and offers a foundation for further research and breeding efforts focused on enhancing the nutritional value of chickpea.