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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Publications at this Location » Publication #408796
Research Project: Improved Pest and Disease Control for Enhanced Woody Perennial Tree Crop and Grapevine Production

Location: Crops Pathology and Genetics Research

Title: First complete genome sequence resource of a Potyvirus lactucae isolate from the United States of America

Author
item REDDY, KISHOREKUMAR - University Of California, Davis
item KUNDA, DIVYA - US Department Of Agriculture (USDA)
item GILBERTSON, ROBERT - University Of California, Davis
item MCDONALD, KAREN - University Of California, Davis
item NANDI, SOMEN - University Of California, Davis
item Sudarshana, Mysore

Submitted to: PhytoFrontiers
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/19/2024
Publication Date: 9/14/2024
Citation: Reddy, K., Kunda, D., Gilbertson, R.L., McDonald, K.A., Nandi, S., Sudarshana, M.R. 2024. First complete genome sequence resource of a Potyvirus lactucae isolate from the United States of America. PhytoFrontiers. 4(4):828-831. https://doi.org/10.1094/PHYTOFR-04-24-0043-A.
DOI: https://doi.org/10.1094/PHYTOFR-04-24-0043-A

Interpretive Summary: This manuscript describes the genome sequence of a lettuce mosaic virus (LMV) isolate from the Salinas Valley in California and its genetic relationship with LMV isolates reported from elsewhere. The genome of this virus isolate was determined for a project aimed at making a plant virus vector to produce therapeutic proteins in plants. The sequence was determined from RNA-Seq as well as from overlapping amplicons generated by RT-PCR. The California isolate of LMV is closely related to an LMV isolate from Chile.

Technical Abstract: Lettuce mosaic virus (LMV) is a devastating pathogen that impacts commercial lettuce production. The complete genome sequence of an LMV isolate from a romaine lettuce plant (LMV-R) collected in 1994 in the Salinas Valley of California was determined by RNA-seq, Sanger sequencing followed by 5' RACE. The genome of LMV-R consists of 10,080 nucleotides and shares the highest nucleotide identity with the LMV isolate CL208 from Chile (97.2%) (GenBank accession KJ161176.1). LMV-R genome encodes for a single large polypeptide (UED15646) with putative proteolytic cleavage sites and showed 98.9% aa identity to a Turkish isolate (UEP55410) and, 98.71% to Chile isolate (AIB00275). The sequence and phylogenetic analysis highlight the close association between LMV-R from the USA and CL208 of Chile, indicating that LMV-R might have been exchanged between North America and South America through international trade.