Skip to main content
ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Fiber Bioscience and Utilization Research » Research » Publications at this Location » Publication #408192
Research Project: Genomic Dissection and Molecular Characterization of Fiber Quality Traits for Cotton Variety Improvement

Location: Cotton Fiber Bioscience and Utilization Research

Title: Interaction of the cyclin-dependent kinase inhibitor GhKRP6 with RING-Type E3 ligase influences the fiber length in upland cotton

Author
item NAOUMKINA, MARINA
item THYSSEN, GREGORY
item ZENG, LINGHE
item SHOCKEY, JAY
item NEUMANN, NICHOLAS - OAK RIDGE NATIONAL LABORATORY
item FLORANE, CHRISTOPHER
item Jenkins, Johnie
item McCarty, Jack
item FANG, DAVID

Submitted to: Industrial Crops and Products
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/2/2024
Publication Date: 12/15/2024
Citation: Naoumkina, M.A., Thyssen, G.N., Zeng, L., Shockey, J.M., Neumann, N., Florane, C.B., Jenkins, J.N., Mccarty Jr, J.C., Fang, D.D. 2024. Interaction of the cyclin-dependent kinase inhibitor GhKRP6 with RING-Type E3 ligase influences the fiber length in upland cotton. Industrial Crops and Products. 222(3) Article 119789. https://doi.org/10.1016/j.indcrop.2024.119789.
DOI: https://doi.org/10.1016/j.indcrop.2024.119789

Interpretive Summary: Upland cotton is the major crop that provides renewable fiber for the textile industry. The length of cotton lint fiber directly affects the quality of the yarn. The ultimate goal of cotton breeding is to improve fiber traits without reducing fiber yield. Eleven cotton cultivars with diverse fiber quality traits have been crossed to develop a population for genetics research and breeding. A genome-wide association study of this population identified a locus on Chromosome D11 (FLD11) associated with fiber length. This study aims to understand how the FLD11 regulates fiber length. We found an interaction between genes from FLD11 and an additional locus on chromosome D12 that influences the fiber length.

Technical Abstract: Upland cotton (Gossypium hirsutum L.) is the major crop that provides renewable fiber for the textile industry. Cotton fiber length is an economically important trait that affects the quality of the yarn. The ultimate goal of cotton breeding is to improve fiber traits without reducing fiber yield. The Upland cotton multi-parent advanced generation inter crosses (MAGIC) population was developed for genetics research and breeding. A genome-wide association study of the MAGIC population identified a significant fiber length QTL on Chromosome D11 (qFL-D11-1). However, how the qFL-D11-1 regulates fiber length is unclear. We fragmented the qFL-D11-1 to evaluate the allelic effect of the candidate genes on fiber length. We observed a strong influence on fiber phenotypes of the recombinant haplotypes from another FL QTL on Chr. D12 (qFL-D12-1). Correlation analysis of the expression patterns of genes from two QTLs with the fiber length of the MAGIC population showed the highest correlation of the RING-type ubiquitin E3 ligase (GhUbE3) from qFL-D12-1. A candidate gene from the qFL-D11-1, encoding KIP-related protein 6 (GhKRP6), exists in two allelic variants: the variant with a reference allele was associated with longer cotton fiber, whereas the variant with an alternative allele was associated with shorter fiber. A yeast two-hybrid assay demonstrated a physical interaction between GhUbE3 (Ghir_D12G006080) and two isoforms of GhKRP6 (Ghir_D11G020340). Analyses of transcriptional activity of the GhUbE3 in developing cotton fiber suggested that the GhUbE3 regulates the dose of inhibitory activity of the GhKRP6 and, therefore, the duration of fiber elongation.