|DAHAN, JENNIFER - University Of Idaho|
|ORELLANA, GARDENIA - University Of Idaho|
|KARASEV, ALEXANDER - University Of Idaho|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2023
Publication Date: N/A
Technical Abstract: While studying grapevine virome in the State of Idaho, five virus genomes were identified as endornaviruses through the use of high-throughput sequencing (HTS) of total RNA samples extracted from two wine grape cultivars. One was determined to be a local isolate of grapevine endophyte endornavirus (GEEV) found in a declining Chardonnay, and four others represented two novel endornaviruses named grapevine endornavirus 1 (GEV1) and grapevine endornavirus 2 (GEV2). All virus genomes spanned a large, single open reading frame encoding polyproteins with easily identifiable helicase and RNA dependent RNA polymerase (RdRP) domains, with GEV2 polyprotein also containing a glycosyltransferase domain. The amino acid sequence of the RdRP domain of GEV1 exhibited the closest affinity to the RdRP of GEEV. GEV2 was found as three genetic variants exhibiting 92.0-99.8% nucleotide sequence identity between each other. In phylogenetic analyses, RdRP and HEL domains of GEV1 and GEV2 polyproteins were placed in two separate clades inside the large lineage of alphaendornaviruses showing affinity to GEEV and Phaseolus vulgaris endornavirus 1, respectively.