Location: Natural Products Utilization ResearchTitle: Metabolomic evaluation of soil Streptomyces sp. for the discovery of novel biopesticidal constituents
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/17/2023
Publication Date: 8/15/2023
Citation: Kim, S., Estep Iii, A.S., Tamang, P., Cantrell, C.L. 2023. Metabolomic evaluation of soil Streptomyces sp. for the discovery of novel biopesticidal constituents. Meeting Abstract. National Meeting of the American Chemical Society, Denver, CO.
Technical Abstract: The emergence of resistance of pathogenic microorganisms to synthetic pesticides commonly employed has prompted a search for innovative natural product-based solutions. One such solution involves exploring and utilizing microorganisms as sources of new biochemical biopesticides and/or microbial biopesticides. Among the plethora of bacterial species in the soil, Streptomyces sp. have been a subject of interest owing to their ubiquitous nature and indispensable role in the decomposition of organic matter and their potential to serve as a source of eco-friendly biopesticides with antifungal and mosquito larvicidal properties. Our research targets previously unexplored Streptomyces species and their secondary metabolites in Aedes aegypti vector control. In our recent report, we identified S. distallicus and S. orinoci as promising biopesticides against Ae. aegypti and indicated that this species, along with its extracts, presents a potential source of microbial biolarvicides. Furthermore, this study evaluated six additional Streptomyces species, namely, S. ardus, S. kentuckensis, S. eurocidicus, S. salmonis, S. hachijoensis, and S. septatus, which are related to S. distallicus at the DNA level, for their possible application as microbial biopesticides, plant protectants, or biochemical biopesticide precursors with agricultural implications. We subjected the culture filtrates of these Streptomyces species to ethyl acetate extraction and conducted a comprehensive analysis using a variety of chromatographic, spectrometric, and spectroscopic techniques. We then subjected the resulting potent bioactive compounds to various in-house assays to evaluate their effectiveness against phytopathogenic fungi and larvae of Ae. aegypti. Further studies are required to identify the specific bioactive molecules, delineate their chemical biosynthetic pathways, and conduct further physiological analyses.