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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Publications at this Location » Publication #402567
Research Project: Advancing Knowledge of the Biology and Etiology of Bacterial Plant Pathogens Towards Management Strategies

Location: Emerging Pests and Pathogens Research

Title: Impact of a carbonic anhydrase on expression of virulence-related genes in Pseudomonas syringae

Author
item ZHANG, WEI - Cornell University
item WAN, LINGWEI - Cornell University
item Stodghill, Paul
item Filiatrault, Melanie

Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/14/2023
Publication Date: 8/20/2023
Citation: Zhang, W., Wan, L., Stodghill, P., Filiatrault, M.J. 2023. Impact of a carbonic anhydrase on expression of virulence-related genes in Pseudomonas syringae. International Congress of Plant Pathology Abstracts and Proceedings. International Congress of Plant Pathology.

Interpretive Summary:

Technical Abstract: Pseudomonas syringae pv. tomato DC3000 (Pto DC3000) is a plant pathogenic bacterium that infects tomato and Arabidopsis. During infection bacteria produce a number of proteins, including those of the Type III secretion system (T3SS) to suppress the host defense response and promote disease. Previously we found that deletion of a Pto DC3000 carbonic anhydrase, cynT, reduced disease symptoms and bacterial population in tomato and resulted in a delay in a hypersensitive response (HR) in N. benthamiana. To further investigate the role of the cynT in Pto DC3000, we performed a global transcriptome analysis for the 'cynT and wild-type (WT) strains in different media. The analysis revealed opposite patterns of expression for some T3SS genes in the cynT mutant compared to the WT when calcium was present. The RNA-Seq data also showed that many genes that encode proteins involved in motility were down regulated in the cynT mutant compared to WT. To investigate regulatory mechanisms involved in expression of cynT, we evaluated expression of cynT in multiple mutant backgrounds. T3SS-related genes such as hrpS, hrpL, hrpJ, and hrpP, were found to impact expression of the cynT gene, suggesting that CynT is a key regulator for interaction with host plants. More research is being conducted to determine how CynT affects the pathogenesis.