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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Publications at this Location » Publication #402565
Research Project: Advancing Knowledge of the Biology and Etiology of Bacterial Plant Pathogens Towards Management Strategies

Location: Emerging Pests and Pathogens Research

Title: Identification of virulence related genes regulated by a bacterial carbonic anhydrase

Author
item WAN, LINGWEI - Cornell University
item ZHANG, WEI - Cornell University
item Karp, Mary
item Stodghill, Paul
item Filiatrault, Melanie

Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/14/2023
Publication Date: 8/20/2023
Citation: Wan, L., Zhang, W., Karp, M.A., Stodghill, P., Filiatrault, M.J. 2023. Identification of virulence related genes regulated by a bacterial carbonic anhydrase. International Congress of Plant Pathology Abstracts and Proceedings. International Congress of Plant Pathology Abstracts.

Interpretive Summary:

Technical Abstract: cynT (PSPTO_5255) encodes a carbonic anhydrase whose expression is induced by calcium in Pseudomonas syringae pv. tomato DC3000. Previously we showed cynT contributes to growth of DC3000 and symptom development in tomato plants. To understand the mechanism of how cynT impacts the virulence of DC3000, global transcriptome analysis (RNA-Seq) was performed for the cynT mutant and wild-type (WT). The expression of many virulence-related genes was impacted when cynT was deleted. On rich media (nutrient broth agar) supplemented with calcium, 73 genes were upregulated, and 56 genes were downregulated in the 'cynT strain compared to the WT. On minimal media (mannitol-glutamate agar) supplemented with calcium, 495 genes were upregulated, and 253 genes were downregulated in the 'cynT strain compared to the WT. Comparing the datasets, we found that the regulation of 31 genes by cynT was independent of the growth condition, however many of these genes showed opposite patterns of expression, including PSPTO_2870 and PSPTO_2871. PSPTO_2870 and PSPTO_2871 are predicted orthologs of putative virulence genes, srfA, and srfB respectively in P. syringae pv. syringae B728. Little is known about the functions of these genes in DC3000. Knockout mutants of srfABC operon were constructed in DC3000 and currently being characterized. The results indicate that the carbonic anhydrase, CynT is a key regulator for bacterial factors related to virulence.