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Research Project: Impact of Maternal Influence and Early Dietary Factors on Child Growth, Development, and Metabolic Health

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Title: Beta-adrenergic agonist induces unique transcriptomic signature in inguinal white adipose tissue

Author
item PAZ, HENRY - University Arkansas For Medical Sciences (UAMS)
item PILKINGTON, ANNA-CLAIRE - University Arkansas For Medical Sciences (UAMS)
item LOY, HANNAH - University Arkansas For Medical Sciences (UAMS)
item ZHONG, YING - University Arkansas For Medical Sciences (UAMS)
item SHANKAR, KARTIK - University Of Colorado
item WANKADE, UMESH - University Arkansas For Medical Sciences (UAMS)

Submitted to: Physiological Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/28/2023
Publication Date: 3/26/2023
Citation: Paz, H., Pilkington, A., Loy, H., Zhong, Y., Shankar, K., Wankade, U. 2023. Beta-adrenergic agonist induces unique transcriptomic signature in inguinal white adipose tissue. Physiological Reports. 11(6):e15646. https://doi.org/10.14814/phy2.15646.
DOI: https://doi.org/10.14814/phy2.15646

Interpretive Summary: This study looked at the way that different types of fat tissue in mice respond to a drug called CL316,243. We used a technique called RNA sequencing to study the genes that were active in each type of tissue. We show that the beige fat had a unique pattern of gene expression that was different from both brown and white fat. These newly identified genes will help us understand complex changes that could happen in obesity, knowledge of which can be used in devising strategy to prevent the obesity and related complications.

Technical Abstract: Activation of thermogenic adipose tissue depots has been associated with improved metabolic health and weight loss. Thermogenic fats could provide means to support efforts towards combating obesity and its associated disorders; however, the molecular drivers that regulate their thermogenic responses still require further clarification. To profile the transcriptome of various adipose tissue types, we collected brown adipose tissue (BAT), gonadal white adipose tissue (gWAT), and inguinal white adipose tissue (iWAT) from 21-week-old male mice that were treated with vehicle or CL316,243 (CL) and performed RNA-Seq. We used iWAT from CL-treated mice for beige fat (iWATCL). Principal coordinate analysis revealed a divergent transcriptional profile in beige adipose compared to BAT and white depots. Differentially expressed genes (DEGs) in beige adipose were associated with the upregulation of pathways involved in cellular immune responses which was similar to iWAT, but also with the upregulation of the browning pathway which was similar to BAT. We identified 14 and 25 DEGs that were specifically upregulated and downregulated in beige adipose, respectively. Overall results indicate transcriptional heterogeneity across adipose tissues and show genes that are specifically regulated in beige adipose that may aid to identify novel pathways involved in the browning/beiging process.