Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #400873
Research Project: Intestinal Microbial Ecology and Non-Antibiotic Strategies to Limit Shiga Toxin-Producing Escherichia coli (STEC) and Antimicrobial Resistance Transmission in Food Animals

Location: Food Safety and Enteric Pathogens Research

Title: Pseudotemporal and spatial organization of B cell activation and differentiation in Peyer’s patches

Author
item WIARDA, JAYNE - Oak Ridge Institute For Science And Education (ORISE)
item Shircliff, Adrienne
item Stasko, Judith
item Ackermann, Mark
item SIVASANKARAN, SATHESH - Iowa State University
item TUGGLE, CHRISTOPHER - Iowa State University
item Loving, Crystal

Submitted to: American Association of Immunologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 2/24/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Organized lymphoid tissues are important sites of immune induction where B cells activate and differentiate. High-resolution transcriptomics have revolutionized current understandings of B cell activation and differentiation in organized lymphoid tissues such as lymph nodes but have not been used to dissect B cell activation and differentiation in Peyer’s patches, important sites of intestinal immune induction. We utilized single-cell RNA sequencing and spatial transcriptomics to define pseudotemporal and spatial organization, respectively, of B cell activation and differentiation in porcine jejunal and ileal Peyer’s patches (JPPs and IPPs). IPPs had greater follicular mass and more defined follicular borders than JPPs, resulting in greater follicular B cell abundance in IPPs and non-follicular B cell abundance in JPPs. Despite follicular morphology and B cell abundances varying between JPPs and IPPs, pseudotemporal trajectories and predicted spatial locations of B cell activation and differentiation were similar between intestinal locations. Transition of resting into follicular B cells was demarcated by increased expression of follicle- and cell cycle-associated genes and movement of cells from interfollicular zones (IFZs) into follicles. Another trajectory indicated transition of resting B cells located in IFZs directly into antibody-secreting cells located in IFZs and crypts, suggesting a route of extrafollicular B cell activation and differentiation. Altogether, results indicate conserved patterns of both follicular and extrafollicular B cell activation and differentiation in Peyer’s patches across intestinal locations, despite differences in cellular abundances and follicular morphology.