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ARS Home » Northeast Area » Frederick, Maryland » Foreign Disease-Weed Science Research » Research » Publications at this Location » Publication #398597
Research Project: Developing Genomic and Biological Resources to Characterize, Diagnose and Detect Emerging and Invasive Vectored Bacterial and Viral Plant Pathogens for Safeguarding U.S. Agriculture

Location: Foreign Disease-Weed Science Research

Title: Draft genome sequences of two Xylella fastidiosa subsp. pauca strains isolated from citrus

Author
item Rogers, Elizabeth
item Sechler, Aaron

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/15/2025
Publication Date: 4/30/2025
Citation: Rogers, E.E., Sechler, A.J. 2025. Draft genome sequences of two Xylella fastidiosa subsp. pauca strains isolated from citrus. Microbiology Resource Announcements. https://journals.asm.org/doi/10.1128/mra.01046-24.
DOI: https://doi.org/10.1128/mra.01046-24

Interpretive Summary: Xylella fastidiosa is a bacterium that grows in parts of the circulatory system of plants, blocking the flow of water and nutrients. It causes diseases in more than 100 different plants which are primarily characterized by dead leaf edges, leaf and fruit drop, and plant death. A Xylella strain from citrus was the first plant pathogen to ever have its genome completely sequenced. However, Xylella is difficult to grow in a laboratory and to preserve in long-term storage. To our knowledge, the sequenced strain, 9a5c, is no longer alive in any laboratory in the United States or Europe. Here we report the genome sequences of three Xylella strains very similar to 9a5c. Two strains were isolated from citrus and one from coffee. These strains are alive in our laboratory and a resource for the international scientific community.

Technical Abstract: Xylella fastidiosa is a xylem-limited plant pathogen that infects more than 100 species of plants. Here, we present draft genomes of two Xylella fastidiosa subsp. pauca strains isolated from citrus and one from coffee. These three strains are a resource for studying citrus variegated chlorosis and coffee leaf scorch that are readily available to the international scientific community.